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Streptomyces nodosus Trejo

14899

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Streptomyces nodosus strain SC 2388 is a whole-genome sequenced bacterial type strain that was isolated in Venezuela from soil from the Orinoco River. This strain produces amphotericin A.
Product category
Bacteria
Strain designation
SC 2388 [ATCC 23942, CBS 926.68, IFO 12895, IMRU 3694, ISP 5109, M 4575, RIA 1123]
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Soil from Orinoco River
Geographical isolation
Venezuela
Applications
Antibiotic production
Product format
Freeze-dried
Storage conditions
2°C to 8°C
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Produces amphotericin A
Produces amphotericin B fungizone
Preceptrol
No

Characteristics

Technical information
ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.

Handling information

Medium
Temperature
26°C
Atmosphere
Aerobic
Handling procedure
  1. Open vial. 
  2. Using a single tube of #1877 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette.  Rehydrate the entire pellet.
  3. Aseptically transfer this aliquot back into the broth tube.  Mix well.
  4. Use several drops of the suspension to inoculate a #196 agar slant and/or plate.
  5. Incubate the tubes and plate at 26°C for 7 days.
Handling notes

Additional information on this culture is available on the ATCC web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Streptomyces nodosus Trejo
Depositors
E.R. Squibb & Sons, Inc.
Patent depository
This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC.  As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
Patent number
2,908,611
Type of isolate
Environmental
Cross references
GenBank AJ250538 Streptomyces nodosus csp1 gene for Csp1 (cold-shock like protein).
GenBank AF114033 Streptomyces nodosus rrnB operon 16S ribosomal RNA, 23S ribosomal RNA, and 5S ribosomal RNA, complete sequence.
GenBank AF114034 Streptomyces nodosus rrnD operon 16S ribosomal RNA, 23S ribosomal RNA, and 5S ribosomal RNA, complete sequence.
GenBank AF114035 Streptomyces nodosus rrnE operon 16S ribosomal RNA, 23S ribosomal RNA, and 5S ribosomal RNA, complete sequence.
GenBank AF114036 Streptomyces nodosus rrnF operon 16S ribosomal RNA, 23S ribosomal RNA, and 5S ribosomal RNA, complete sequence.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Disclosures
This material is cited in a US and/or international patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Depositor of the party to which the material was furnished.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Dutcher JD, et al. Amphotericin B, its production and its salts. US Patent 2,908,611 dated Oct 13 1959

Dutcher JD, et al. Amphotericin A and its salts. US Patent 2,908,612 dated Oct 13 1959

Trejo W, Bennett RE. Streptomyces nodosus sp. n., the amphotericin-producing organism. J. Bacteriol. 85: 436-439, 1963. PubMed: 13994057

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

Shirling EB, Gottlieb D. Cooperative description of type cultures of Streptomyces. III. Additional species descriptions from first and second studies. Int J Syst Bacteriol 18: 279-392, 1968.

View All Curated Citations for this Product

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