Methylobacterium rhodinum (Heumann) Green and Bousfield
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2. From a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate an additional broth tube, a #3 agar slant and/or a plate.
5. Incubate the tubes and plate at 26°C for 24 to 48 hours.
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Green PN, Bousfield IJ. Emendation of Methylobacterium Patt, Cole, and Hanson 1976; Methylobacterium rhodinum (Heumann 1962) comb. nov. corrig.; Methylobacterium radiotolerans (Ito and Iizuka 1971) comb. nov. corrig.; and Methylobacterium mesophilicum (Austin and Goodfellow 1979) comb. nov.. Int. J. Syst. Bacteriol. 33: 875-877, 1983.
Urakami T, Komagata K. Protomonas, a new genus of facultatively methylotrophic bacteria. Int. J. Syst. Bacteriol. 34: 188-201, 1984.
Mol. Gen. Genet. 197: 425-436, 1984.
Heumann W. Die methodik der Keruzung Sternbildender bacterien. Biol. Zentralbl. 81: 341-354, 1962.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.