Salmonella enterica subsp. enterica serovar Typhimurium GFP
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Important: a concentration of 100 µg/mL ampicillin must be maintained at all time during culture. Absence or low concentrations of ampicillin will result in plasmid loss. This strain is stable for up to 5 consecutive passages in the recommended culture conditions.
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Barbier M and Mittar D. Novel fluorescent reporters for studying pathogen-host interactions. ASM general meeting 2014 Poster.
Choi KH, Schweizer HP. mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Nat. Protoc. 1(1): 153-161, 2006. PubMed: 17406227