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Klebsiella pneumoniae subsp. pneumoniae (Schroeter) Trevisan


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Klebsiella pneumoniae subsp. pneumoniae strain NCTC 9633  is a bacterial type strain with applications in antimicrobial preservative testing, bioresistance testing, media testing, and quality control.
Product category
Product type
Preceptrol culture
Strain designation
NCTC 9633 [NCDC 298-53, NCDC 410-68]
Type strain
Food testing
Infectious disease research
Media testing
Quality control
Respiratory disease research
Product format
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Assay of antimicrobial preservatives
Bacterial resistance testing adhesives
Bioresistance testing
Media testing
Quality control strain
Quality control strain for Abbott, API and bioMerieux Vitek, IDS, and Sensititre products
This strain is recommended by ATCC for use in the tests described in ASTM Standard Test Methods D4783-89 and E979-91 where only the taxon is specified.
Food testing
Respiratory research

Recommended as a test microorganism for ASTM E2275: Standard Practice for Evaluating Water-Miscible Metalworking Fluid Bioresistance and Antimicrobial Pesticide Performance




Handling information

Handling procedure
  1. Open vial according to enclosed instructions.
  2. Using a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
  3. Aseptically transfer this aliquot back into the broth tube. Mix well.
  4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate.
  5. Incubate all tubes and plate at 37°C for 24 hours.
Handling notes
Additional information on this culture is available on the ATCC® web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Klebsiella pneumoniae subsp. pneumoniae (Schroeter) Trevisan
Chain of custody
ATCC <-- NCTC <-- NCDC 298-53
Cross references
GenBank X87284 K.pneumoniae 23S rRNA gene, ATCC 13883 T.
GenBank Y11487 K.pneumoniae ribosomal RNA, strain ATCC13883.
GenBank AX109584 Sequence 317 from Patent WO0123604.
GenBank AX111035 Sequence 1768 from Patent WO0123604.
GenBank AX111049 Sequence 1782 from Patent WO0123604.
GenBank AX111221 Sequence 1954 from Patent WO0123604.
GenBank AX111487 Sequence 2220 from Patent WO0123604.
GenBank AX111508 Sequence 2241 from Patent WO0123604.
GenBank AX111528 Sequence 2261 from Patent WO0123604.
GenBank AF190857 Klebsiella pneumoniae cloacin operon, complete sequence.
GenBank U67169 Klebsiella pneumoniae flavodoxin (fldA) gene, complete cds.
GenBank AF038432 Klebsiella pneumoniae DNA gyrase A (gyrA) gene, partial cds.
GenBank AF052258 Klebsiella pneumoniae DNA gyrase A (gyrA) gene, partial cds.
GenBank U77444 Klebsiella pneumoniae RNA polymerase beta-subunit (rpoB) gene, partial cds.
GenBank U14463 Klebsiella pneumoniae ATCC 13883 6-phosphogluconate dehydrogenase (gnd) gene, partial cds.
GenBank M66869 Klebsiella pneumoniae (ATCC 13883) glyceraldehyde-3-phosphate dehydrogenase gene, partial cds.
GenBank L23871 Klebsiella pneumonia flavodoxin (fldA) gene, partial cds, ferric uptake regulation (fur) gene, complete cds, and citrate transport protein (citA) gene, partial cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

Listeria species -- Biochemical Identification Method (Vitek GPI and GNI+). Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 992.19.

Standard Practice for Evaluating Water-Miscible Metalworking Fluid Bioresistance and Antimicrobial Pesticide. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2275-03E01.

RapID™ SS/u QC Set. Thermo Fisher Scientific.

ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions)

View All Curated Citations for this Product

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