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Lactobacillus delbrueckii subsp. bulgaricus (Orla-Jensen) Weiss et al.


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Lactobacillus delbrueckii subsp. bulgaricus strain Lb14 [IAM 12472, IFO 13953] is a whole-genome sequenced bacterial strain isolated from Bulgarian yogurt. This type strain has applications in bioinformatics and DNA hybridization.
Product category
Product type
Preceptrol culture
Strain designation
Lb14 [IAM 12472, IFO 13953]
Type strain
Genome sequenced strain
Isolation source
Dairy products - Bulgarian yogurt
Product format
Storage conditions
2°C to 8°C


ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
DNA hybridization


Genome sequenced strain

Handling information

95% Air, 5% CO2
Handling procedure
  1. Open vial.
  2. Using a single tube of #416 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
  3. Aseptically transfer this aliquot back into the broth tube. Mix well.
  4. Use several drops of the suspension to inoculate a #416 agar slant and/or plate.
  5. Incubate the tubes and plate at 37°C in an atmosphere of 5% CO2 for 24 to 48 hours. Loosen screw caps of all test tubes during the incubation period.
Handling notes
This culture may require growth to be established in broth before growth on agar can be achieved. Best growth in broth is at 16-18 hours for harvesting.
Purified genomic DNA of this strain is available (ATCC 11842D-5).
Additional information on this culture is available on the ATCC web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Lactobacillus bulgaricus (Orla-Jensen) Rogosa and Jensen
PA Hansen
Chain of custody
ATCC <-- PA Hansen <-- S Orla-Jensen 14 (Thermobacterium bulgaricum)
Type of isolate
Food & Beverage
Cross references
GenBank X89439 L.delbrueckii DNA for AsnS2 gene.
GenBank X79114 L.delbrueckii (ATCC 11842) ISL3 gene.
GenBank X61190 L.delbrueckii nifS-like gene (partial).
GenBank X89438 L.delbrueckii DNA for AsnA and AsnS1 genes.
GenBank Z75477 L.bulgaricus rrn operon, 23S-5S rRNA spacer.
GenBank Z75476 L.bulgaricus rrn operon, 16S-23S rRNA spacer (short).
GenBank Z75475 L.bulgaricus rrn operon, 16S-23S rRNA spacer (long), tRNA-Ile and tRNA-Ala genes.
GenBank AY040213 Lactobacillus delbrueckii subsp. bulgaricus ISL4 transposase gene, complete cds.
GenBank M55068 L.delbrueckii bulgaricus lactose permease and beta-galactosidase (lacZ) genes, complete cds.
GenBank AY040218 Lactobacillus delbrueckii subsp. bulgaricus ISL5, partial sequence; and ISL4 transposase gene, complete cds.
GenBank AF113602 Lactobacillus delbrueckii subsp. bulgaricus 16S-23S ribosomal RNA intergenic spacer region, complete sequence.
GenBank X15246 Lactobacillus delbrueckii tRNA-genes transfer RNA-Gly, transfer RNA-Arg, transfer RNA-Glu, transfer RNA-Val, transfer RNA-Asp and 5S ribosomal RNA.
GenBank X15245 Lactobacillus delbrueckii 5S rRNA gene and tRNA-genes for transfer RNA-Asn, transfer RNA-Gly, transfer RNA-Pro, transfer RNA-Arg, transfer RNA-Val, transfer RNA-Asp and 5S ribosomal RNA.
GenBank CR954253 Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 complete genome.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

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Frequently Asked Questions


Curated Citations

Rogosa M, Hansen PA. Nomenclatural considerations of certain species of Lactobacillus Beijerinck. Int. J. Syst. Bacteriol. 21: 177-186, 1971.

Simonds J, et al. Deoxyribonucleic acid hybridization among strains of lactobacilli. J. Bacteriol. 107: 382-384, 1971. PubMed: 4935327

Kim SI, et al. Lactobacillus bulgaricus asparagine synthetase and asparaginyl-tRNA synthetase: coregulation by transcription antitermination. J. Bacteriol. 178: 2459-2461, 1996. PubMed: 8636057

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions)

View All Curated Citations for this Product

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