Streptomyces fradiae (Waksman and Curtis) Waksman and Henrici
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2. Using a single tube of #1877 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #527 agar slant and/or plate.
5. Incubate the tubes and plate at 26oC for 7 to 10 days.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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Crameri R, Davies JE. Increased production of aminoglycosides associated with amplified antibiotic resistance genes. J. Antibiot. 39: 128-135, 1986. PubMed: 3949626
Ettlinger L, et al. Zur Systematik der Actinomyceten. 4. Eine arteinteilung der Gattung Streptomyces Waksman et Henrici. Arch. Mikrobiol. 31: 326-358, 1958.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.
Waksman SA, Lechevalier HA. Neomycin, a new antibiotic active against streptomycin resistant bacteria, including tuberculosis organisms. Science 109: 305-307, 1949.
Waksman SA, et al. The in vivo activity of neomycin. J. Bacteriol. 58: 229-237, 1949.