Bovine viral diarrhea virus 1
VR-1422 ™
Download Genome Learn about the ATCC Genome Portal- Product category
-
Viruses
- Product type
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Animal virus
- Classification
- Flaviviridae, Pestivirus
- Strain designation
- NADL (biologically cloned)
- Common name
- Bovine viral diarrhea virus 1
- Genome sequenced strain
- Yes
- Isolation source
- Clinical specimen- animal.
- Applications
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Agricultural research
- Product format
- Frozen
- Storage conditions
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-70°C or colder
Documentation
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Detailed product information
Characteristics
- Mycoplasma contamination
- Not detected
Handling information
- Host
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Host Range: cattle, rabits, sheep, swine
- Effects
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CPE; cytoplasmic vacuolation
- Complete medium
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EMEM (ATCC® 30-2003™) + 10% Horse Serum (ATCC® 30-2041™)
- Temperature
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37°C
- Atmosphere
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95% Air, 5% CO2
- Incubation
- 2 to 6 days at 37°C, a 5% CO2 in air atmosphere is recommended.
- Recommendation for infection
- For best results cells should be 24 to 48 hours old and 80%-90% confluent (not 100% confluent).
- Handling notes
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This high titer strain is the same as ATCC® VR-534TM (NADL strain) except for passage history and its amenability to be grown to high titer.
- Key abbreviations
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bp, Base pairsBT, Bovine turbinate cellsBVD, Bovine viral diarrheaBVDV, Bovine viral diarrhea virusCO2, Carbon dioxideCPE, Cytopathic effectEMEM, Eagle's Minimum Essential MediumFA, Fluorescent antibody assayFBS, Fetal bovine serumμM, MicromolarNADL, National Animal Disease LaboratoryNCBI, National Center for Biotechnology InformationPCR, Polymerase chain reactionPen, PenicillinRT-PCR, reverse-transcriptase polymerase chain reactionStrep, StreptomycinTC, Tissue cultureTCID[50], Median tissue culture infective doseVMRD, Veterinary Medical Research and Development
Quality control specifications
- Verification method
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Whole-genome Sequencing
History
- Deposited as
- Bovine viral diarrhea
- Depositors
- SR Bolin
- Type of isolate
- Animal
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
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The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
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This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
United States Veterinary Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors
For every order of this item, you must provide a valid Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors (VS Form 16-6A) obtained from the United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service. We cannot ship this item until we receive this permit.
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Frequently Asked Questions
References
Curated Citations
Gutekunst KE. . Can. J. Comp. Med. Vet. Sci. 27: 121, 1963.
Obtained from spleen of a naturally occurring fatal case of BVD in the closed herd at National Animal Disease Laboratory, Iowa, 1962. ATCC VR-1422 is the same as ATCC VR-534 but is described by the contributer as having the potential to be grown to higher titers.
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