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TransfeX™ Transfection Reagent (ATCC® ACS-4005™)

Permits and Restrictions

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Product Format 500 µL
Storage Conditions Store at 2°C to 8°C
Shipping Information Refrigerated Bricks
Applications Transient transfection, stable transfection, gene expression, protein production, and genetic manipulation.
Comments

Cationic lipid compound contained in an aqueous based solution. Reagent is designed for transfection of primary cells and hard-to-transfect cell lines.  TransfeX Transfection Reagent provides high efficiency with low cell mortality. Cell line-specific protocols can be found here.
Biosafety Level N/A
Quality Control Specifications The molecular weight was determined by Mass Spectroscopy to be consistent with the expected structure.
Sterility Testing No evidence  of bacterial or fungal contamination. Mycoplasma testing was negative for the presence of mycoplasma.
Functional Testing HeLa cells are transfected with a lacZ reporter plasmid. The peak activity and the cytotoxicity for the test lot is determined in a beta-galactosidase functional assay and compared to control lot.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS
Other Documentation
Other Document
TransfeX List of Cell-Line Specific Protocols Other Document
TransfeX Instructions for Use
Restrictions

This product's use is governed by a Limited Use Label License. For information on obtaining a license to use this product for purposes other than those permitted in the Limited Use Label License, please contact the ATCC Licensing Group at licensing@atcc.org.
FAQs
  1. Precipitation in TransfeX Transfection Reagent
    Occasionally a precipitate may form in the TransfeX transfection reagent (ACS-4005). Cold temperatures may cause the lipid to precipitate out. To get the precipitate back into solution, simply wa...
    Date Updated: 8/1/2019
References

Yang R, et al. Cic loss promotes gliomagenesis via aberrant neural stem cell proliferation and differentiation. Canc Res: canres-1018, 2017. PubMed: 28939681

Marczak MM, et al. Circadian rhythmicity: A functional connection between differentiated embryonic chondrocyte-1 (DEC1) and small heterodimer partner (SHP). Arch Biochem Biophys 631: 11-18, 2017. PubMed: 28797635

Perrin A, et al. Increased expression of laminin subunit Alpha 1 Chain by dCas9-VP160. Mol Ther Nucleic Acids 6: 68-79, 2017. PubMed: 28325301

McBride JD, et al. Bone marrow mesenchymal stem cell-derived CD63+ exosomes transport Wnt3a exteriorly and enhance dermal fibroblast proliferation, migration, and angiogenesis in vitro. Stem Cells Dev 26(19): 1384-1398, 2017. PubMed: 28679315

Schuler MH, et al. Miro1-mediated mitochondrial positioning shapes intracellular energy gradients required for cell migration. Mol Biol Cell 28(16): 2159-2169, 2017. PubMed: 28615318

Groff AF, et al. In vivo characterization of Linc-p21 reveals functional cis-regulatory DNA elements. Cell Rep 16(8): 2178-2186, 2016. PubMed: 27524623

Cheung J, et al. A nonviral vector with transfection activity comparable with adenoviral transduction. Ther Deliv 7(11): 739-749, 2016. PubMed: 27790941

Aguet F, et al. Membrane dynamics of dividing cells imaged by lattice light-sheet microscopy. Mol Biol Cell 27(22): 3418-3435, 2016. PubMed: 27535432

Sathanoori R, et al. Shear stress modulates endothelial KLF2 through activation of P2X4. Purinergic Signal 11(1): 139-153, 2015. PubMed: 25563726