TransfeX™ Transfection Reagent (ATCC® ACS-4005™) General Information Quality Control Specifications Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits View Restrictions Product Format 500 μL Storage Conditions Store at 2°C to 8°C Shipping Information Refrigerated Bricks Applications Transient transfection, stable transfection, gene expression, protein production, genetic manipulation. Comments Reagent is designed for transfection of primary cells and hard-to-transfect cell lines. TransfeX Transfection Reagent provides high efficiency with low cell mortality. Cell line-specific protocols can be found here. Biosafety Level N/A Quality Control Specifications The molecular weight was determined by Mass Spectroscopy to be consistent with the expected structure. Sterility Testing No evidence of bacterial or fungal contamination. Mycoplasma testing was negative for the presence of mycoplasma. Functional Testing HeLa cells are transfected with a lacZ reporter plasmid. The peak activity and the cytotoxicity for the test lot is determined in a beta-galactosidase functional assay and compared to control lot. Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis SDS Other Documentation TransfeX List of Cell-Line Specific Protocols TransfeX Instructions for Use Restrictions This product's use is governed by a Limited Use Label License. For information on obtaining a license to use this product for purposes other than those permitted in the Limited Use Label License, please contact the ATCC Licensing Group at licensing@atcc.org. FAQs Variable Transfection Reaction Size Yes, TransfeX is suitable for scaling up transfection volumes as long as the same ratio of DNA of TransfeX reagent is maintained. Date Updated: 3/27/2014 Optimize transfection conditions Follow the recommended transfection condition for each cell type. Monitor cellular toxicity at days 1 and 2 compared to untransfected cells. Reduce the a...Date Updated: 3/27/2014 Transfection media concerns TransfeX has been optimized for intracellular delivery of nuclei acids into cultured...Date Updated: 3/27/2014 Toxicity and transfection We usually see more cellular toxicity at day 2 than at day 1 post-transfection. Reducing the amount of DNA and transfection reagent added to the cells and increasing cell dens...Date Updated: 3/27/2014 Lower than expected transfection efficiency Verify that cells are contaminant-free. Mycoplasma contamination can cause a suppression of transfection efficiency. <...>Date Updated: 3/27/2014 Cell-specific transfection toxicity 3T3-L1 and MDA-MB-231 cells are not as resilient to the reagent and may exhibit cytotoxic effects. Refer to the important guidelines on the cell line specific product sheet wh...Date Updated: 3/27/2014 Different vectors effect transfection efficiency Different promoters work better with certain cell types. CMV, though a strong promoter, may not be optimal for all cell types. For example, EF-1α is a better promo...Date Updated: 3/27/2014