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Influenza A virus (H3N2)

VR-822

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Influenza A virus (H3N2) strain A/Victoria/3/75 is propagated in 10-day-old SPF chicken embryonated eggs. This strain was isolated from a human patient in Melbourne, Australia, and was deposited by AP Kendal. This product is whole-genome sequenced and has applications in influenza research.
Product category
Viruses
Product type
Animal virus
Classification
Orthomyxoviridae, Alphainfluenzavirus
Strain designation
A/Victoria/3/75
Genome sequenced strain
Yes
Isolation source
Isolated from humans.
Geographical isolation
Australia; Victoria; Melbourne
Product format
Frozen
Storage conditions
-70°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

General

Specific applications
Influenza Research

Characteristics

Comments
Hemagglutinin member of H3 subgroup, but antigenically variant from prior strains. Neuraminidase member of N2 subgroup, but antigenically variant from prior strains. Cross-reacts with A/Hong Kong/68, A/England/42/72, A/Port Chalmers/73 and A/Scotland/74 viruses.
Mycoplasma contamination
Not detected

Handling information

Host
10 day old incubated specific pathogen free embryonated chicken eggs
Effects
death of host animal; hemagglutination of red blood cells
Temperature
33°C
Incubation
2-3 days with humidity
Recommendation for infection
Dilute virus with D-PBS prior to inoculation. While candling the eggs, draw a pencil line around the air sac. Using an 18.5 gauge needle, poke a hole into the air sac. Using a 1.0 mL syringe fitted with a 22.5 gauge needle, insert the needle through the hole and into the allantoic cavity. Inject 0.2 mL of diluted inoculum into the allantoic cavity. Seal the hole with nail polish or sterile tape. Incubate eggs at 33°C 2-3 days, checking embryo viability every 12-18 hours and discard non-viable eggs.
To harvest allantoic fluid, refrigerate eggs for at least 2 hour post incubation. Use sterile scissors to cut away the shell around the air sack. Aspirate the allantoic fluid with a syringe or a pipette. Avoid capturing blood or yolk in the allantoic fluid.
Handling notes
Hemagglutinin member of H3 subgroup, but antigenically variant from prior strains. Neuraminidase member of N2 subgroup, but antigenically variant from prior strains. Cross-reacts with A/Hong Kong/68, A/England/42/72, A/Port Chalmers/73 and A/Scotland/74 viruses.
Key abbreviations
Al, Allantoic
°C, Degrees Celsius
CE, Chicken embryo
CEID[50], The Chicken Embryo Infectious Dose 50% endpoint is the 50% infectious endpoint in a chicken embryo.
CRBC, Chicken red blood cells
D-PBS, Dulbecco's Phosphate-buffered saline
H, Hemagglutinin
HA, Hemagglutination
i.a., Intraallantoic
mL, Milliliter
N, Neuraminidase
NIH, National Institutes of Health
RBC, Red blood cell

Quality control specifications

Volume
1.0 mL
Verification method
Whole-genome Sequencing

History

Deposited as
Influenza A virus
Depositors
AP Kendal
Type of isolate
Human

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

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