Yellow Fever Surveillance Kits

The YF MAC-HD is a qualitative assay based on the in-house YF MAC-ELISA method developed by CDC that detects IgM to yellow fever virus. The YF MAC-HD uses anti-human IgM to capture IgM in human serum. If IgM reactive to yellow fever is present, this reacts with non-infectious yellow fever whole virus antigen, which is detected using a horseradish peroxidase-conjugated flavivirus group-reactive monoclonal antibody, and a measurable colorimetric reaction is produced using a 3,3’,5,5’-tetramethylbenzidine substrate. The assay has been adapted to a kit format with a faster completion time and incorporates stability and ease-of-use making it convenient in a wide variety of laboratory settings. The protocol to resolve equivocal results is included in the instruction for use.

The YF MAC-HD is for research use only (RUO) and is intended for use in the detection of anti-yellow fever virus IgM in human serum. It should be used in a laboratory setting by trained laboratory personnel. The results of this assay are qualitative and should be used in the context of laboratory surveillance for yellow fever. Results should be confirmed according to applicable laboratory guidelines. The assay has not been validated for clinical purposes (i.e., the diagnosis of yellow fever disease).