BK polyomavirus (ATCC® VR-837)

Classification: Polyomaviridae, Polyomavirus  /  Product Format: 1 mL per vial

Classification Polyomaviridae, Polyomavirus
Deposited As BK virus
Agent BK polyomavirus
Common Name BK Virus
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 1 mL per vial
Storage Conditions -70°C or colder
Comments Hemagglutination of 0.5% GPRBC.  Virus MUST be freeze-thaw cycled 6 times for greatest infectivity.
Effect on Host
CPE, monolayer degeneration and some cell rounding
Recommended Host
HEL 299 (ATCC CCL-137)
Growth Conditions
Temperature: 37°C
Recommendations For Infection: For best results, cells should be 24 to 48 hours old and 70-90% confluent (not 100% confluent).
Incubation: 14-23 days, a 5% CO2 in air atmosphere is recommended
Effect on Host
CPE, monolayer degeneration and some cell rounding
Name of Depositor G di Mayorca
Source Urine of a kidney transplant patient, London, 1970
References

Gardner SD, et al. New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet 1: 1253-1257, 1971. PubMed: 4104714