Vaccinia virus ts mutant (ATCC® VR-3108)

Classification: Poxviridae, Orthopoxvirus  /  Product Format: frozen

Classification Poxviridae, Orthopoxvirus
Deposited As Vaccinia virus, Orthopoxvirus
Agent Vaccinia virus ts mutant
Strain IHD-W Dts41
Applications
The mutation was assigned by complementation analysis to vaccinia map location E(2-8).
Biosafety Level 2 Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses
Isolation
This conditional lethal mutant, originally designated plaque isolate no. 6606, was isolated from the IHD-W strain of Vaccinia by S. Dales. The IHD-W strain was derived from an IHD-J strain of Vaccinia.
Product Format frozen
Storage Conditions -70°C or colder
Comments
Selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. The non-permissive incubation temperature is 39.5oC. This mutant was assigned a Dales EM category of P, characterized by immature normal particles and mature particles with cores that are predominantly lucent at the center. The mutation was assigned by complementation analysis to vaccinia map location E(2-8).
Effect on Host
Cytopathic effects include enlargement and rounding of cells, followed by death and eventual sloughing.
CPE: enlargement, plaques with rounding and eventual sloughing
Growth Medium ATCC® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum
Recommended Host
Production Host: BSC-40 (ATCC CRL-2761)

Recommended Host: BSC-40 (ATCC CRL-2761)

Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Growth Conditions
1-3 days at 31 C with 5% CO2
Effect on Host
Cytopathic effects include enlargement and rounding of cells, followed by death and eventual sloughing.
CPE: enlargement, plaques with rounding and eventual sloughing
Name of Depositor S Dales, RC Condit
Special Collection NCRR Contract
Chain of Custody
References

Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807

Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746

Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.