Recommended Host
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WI-38 cells (ATCC® CCL-75™)
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Growth Conditions
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Temperature: 33°C
Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 1.0). Adsorb 1.5-2.0 hours at 33°C in a humidified 5% CO2 atmosphere on a rocking platform. End adsorption by adding virus growth medium.
Incubation: 3-5 days at 33°C in a humidified 5% CO2 atmosphere on a rocking platform, until CPE is progressed through 80% of the monolayer.
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