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Human rhinovirus 48 (ATCC® VR-1875™)

Classification: Picornaviridae, Enterovirus, Rhinovirus B  /  Product Format: frozen 1.0 mL per vial

Permits and Restrictions

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Classification Picornaviridae, Enterovirus, Rhinovirus B
Deposited As Rhinovirus 48
Agent Human rhinovirus 48
Strain 1505
Applications Respiratory research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format frozen 1.0 mL per vial
Storage Conditions -70°C or colder
Images VR-1875 images
Comments

ATCC® VR-1875™ was derived from ATCC® VR-515™ following treatment with diethyl ether to remove mycoplasma contamination.

Recognized Reference Strain: Collaborative Report on Rhinoviruses, Nature (Lond.) 213: 761, 1967.
Effect on Host
CPE, refractile rounding and sloughing with cell degeneration
Recommended Host
WI-38 cells (ATCC® CCL-75™)
Growth Conditions

Temperature: 33°C

Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 1.0). Adsorb 1.5-2.0 hours at 33°C in a humidified 5% CO2 atmosphere on a rocking platform. End adsorption by adding virus growth medium.

Incubation: 3-5 days at 33°C in a humidified 5% CO2 atmosphere on a rocking platform, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, refractile rounding and sloughing with cell degeneration
Name of Depositor VV Hamparian, RM Conant, Ohio State University
References

Kapikian A, et al. Characterization of two newly recognized rhinovirus serotypes of human origin. Proc. Soc. Exp. Biol. Med. 122: 1155-1162, 1966. PubMed: 4288216

Kapikian AZ et al. Rhinoviruses: a numbering system. Nature 213: 761-762, 1967. PubMed: 4291698

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS
Other Documentation
Other Document
Micrograph of Host Effects