To ATCC Valued Customers,

ATCC stands ready to support our customers’ needs during the coronavirus pandemic. If you experience any issues with your products or services, please contact ATCC Customer Service at sales@atcc.org. For Technical questions please contact tech@atcc.org. Thank you.
X

Dempsey (ATCC® CCL-28)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  /  Tissue: skin  /  Disease: Klinefelter syndrome

Permits and Restrictions

View Permits

Organism Homo sapiens, human
Tissue skin
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Klinefelter syndrome
Age 1 year 10 months
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype model number = 49; range = 41 to 95
Stability graph shows 100% stability in the stemline number (49). Karyotypes of cells with stemline number of chromosomes were stable with all cells having three extra chromosomes in the X, 6-12 group.
Clinical Data
male
Caucasian
1 year 10 months
Virus Susceptibility Human poliovirus 1
Vesicular stomatitis virus
Comments
Klinefelter Syndrome - XXXXY.
Senesces after approximately 30 passages.
Complete Growth Medium McCoy’s 5a medium (modified) with 1.5 mM L-glutamine adjusted to contain 2.2 g/L sodium bicarbonate, 80%; fetal bovine serum, 20%
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin 0.53mM  EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  5. Add appropriate aliquots of the cell suspension into new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: 1:2 to 1:3
Medium Renewal: Twice per week

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.  

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 11,13
D7S820: 8,12
TH01: 8,9.3
TPOX: 8
vWA: 17,18
Isoenzymes
G6PD, B
Population Doubling Capacity Senesces after approximately 30 passages
Name of Depositor TC Hsu
Deposited As Homo sapiens
Year of Origin April, 1964
References

Huang S, et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J. Virol. 70: 4502-4508, 1996. PubMed: 8676475

Hwu TC, Lockhart LH. The beginning and the terminal stages of DNA synthesis of human cells with an XXXXY constitution. Hereditas 52: 320-324, 1965. PubMed: 5889982

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Huang S, et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J. Virol. 70: 4502-4508, 1996. PubMed: 8676475

Hwu TC, Lockhart LH. The beginning and the terminal stages of DNA synthesis of human cells with an XXXXY constitution. Hereditas 52: 320-324, 1965. PubMed: 5889982

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.