Hs888Lu (ATCC® CCL-211)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  /  Disease: Osteosarcoma

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Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease Osteosarcoma
Age 20 years
Gender male
Ethnicity Caucasian
Applications
The line was derived from normal tissue from patient who had osteosarcoma metastatic to the lung.
Storage Conditions liquid nitrogen vapor phase
Karyotype normal male; diploid; stable
Derivation
The line was derived from normal tissue from patient who had osteosarcoma metastatic to the lung.
Clinical Data
The line was derived from normal tissue from patient who had osteosarcoma metastatic to the lung.
male
Caucasian
20 years
Comments
The line was derived from normal tissue from patient who had osteosarcoma metastatic to the lung.
The tissue was taken from a normal area of lung.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11,14
D16S539: 8,12
D5S818: 13
D7S820: 7,11
THO1: 10,9.3
TPOX: 8,11
vWA: 15,18
Name of Depositor WA Nelson-Rees
Year of Origin August, 1975
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation