Animal testing has been a crucial part in the toxicology evaluation of drugs and chemicals. Recently, however, there has been a shift toward using non-animal in vitro models in place of traditional in vivo animal models. Three-dimensional (3-D) in vitro models are becoming increasingly popular in the toxicology space as the need for these new technologies continues to grow. Here, we outline the different modalities of 3-D dermal culture for toxicology testing as alternatives to animal models.