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Escherichia coli (Migula) Castellani and Chalmers (ATCC® BAA-1427)

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Strain Designations: P1  /  Type Strain: no  /  Biosafety Level: 1

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Verified By Whole-genome Sequencing
Classification Enterobacteriaceae, Escherichia
Strain Designations P1
This is a non-pathogenic E. coli strain that is recommended by the USDA as a surrogate indicator organism to measure changes in microbial counts.
Cattle Hides
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain no
Non-toxigenic isolate.
The depositor states that no cytotoxicity was observed with African green monkey kidney (Vero) cells.
The lack of virulence factor genes has been confirmed by the E. coli  Reference Center of Pennsylvania State University (see virulence gene profile).
Medium ATCC® Medium 18: Trypticase Soy Agar/Broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor JS Dickson, K Marshall
Special Collection ATCC
Chain of Custody ATCC <-- J Dickson, K Marshall
Cattle Hides

JS Dickson, personal communication

Marshall K M, et al. Identification of Escherichia coli O157:H7 Meat processing Indicators for Fresh Meat through Comparison of the Effects of Selected Antimicrobial Interventions. J. Food Prot. 68: 2580-2586, 2005.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
  1. Accessing genome sequencing data

    Date Updated: 9/30/2019

  2. Criteria for reference quality genomes

    Date Updated: 9/30/2019