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Aspergillus niger van Tieghem (ATCC® 9142)

Strain Designations: NRRL 599 [Doelger 2, IMI 41874, X-172]  /  Product Format: freeze-dried

Permits and Restrictions

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Strain Designations NRRL 599 [Doelger 2, IMI 41874, X-172]
Degrades acronine acronycine
Degrades apple pomace
Degrades brewery wastes
Degrades cotton wastes
Degrades inulin
Degrades molasses
Degrades pineapple wastes
Produces 12-hydroxy-trans-nerolidol
Produces 18-homo-19-norcortisone
Produces 18-homo-19-norhydrocortisone
Produces 9-hydroxyacronycine
Produces L-malic acid
Produces citric acid citrate
Produces gluconic acid
Produces grindelane dimers
Produces hydroxygrindelanes
Produces hydroxylated biphenyl compounds
Produces hydroxylated steroids steroids, hydroxylated
Produces lipase
Reduces 3,5-dimethoxycinnamic acid
Reduces 3-methobenzoic acid
Reduces 3-methoxy-8(14)-seco-1,3,5(10),9(11)-estratetraene-14,17-dione
Transforms acronine acronycine
Transforms chromanone
Transforms flavonoids
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Preceptrol® no
Medium ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 323: Malt agar medium
ATCC® Medium 336: Potato dextrose agar (PDA)
Growth Conditions
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence


D1D2 region of the 28S ribosomal RNA gene


beta-tubulin gene


Name of Depositor NRRL
Chain of Custody
ATCC <-- NRRL <-- A.J. Moyer strain Doelger 2

Bercovitz A, et al. Localization of pyruvate carboxylase in organic acid-producing Aspergillus strains. Appl. Environ. Microbiol. 56: 1594-1597, 1990. PubMed: 2383004

Schwartz RD. Microbial production of hydroxylated biphenyl compounds. US Patent 4,153,509 dated May 8 1979

Gadsby B, Greenspan G. C-21 hydroxylation products of steroids. US Patent 3,529,000 dated Sep 15 1970

Gibian H, et al. Production of optically active antipodes. US Patent 3,562,112 dated Feb 9 1971

Arfmann HA, Abraham WR. Microbial reduction of aromatic carboxylic acids. Z. Naturforsch. Sect. C Biosci. 48: 52-57, 1993.

Auret BJ, Holland HL. Microbiological 18-hydroxylation of steroids. J. Chem. Soc. Commun. 1195: 1157, 1971.

Roukas T. Production of citric acid from beet molasses by immobilized cells of Aspergillus niger. J. Food Sci. 56: 878-880, 1991.

Drysdale CR, McKay AM. Citric acid production by Aspergillus niger in surface culture on inulin. Lett. Appl. Microbiol. 20: 252-254, 1995. PubMed: 7766122

Ibrahim AR, Abul-Hajj YJ. Microbiological transformation of chromone, chromanone, and ring A hydroxyflavones. J. Nat. Prod. 53: 1471-1478, 1990. PubMed: 2089118

Tran CT, et al. Selection of a strain of Aspergillus for the production of citric acid from pineapple waste in solid-state fermentation. World J. Microbiol. Biotechnol. 14: 399-404, 1998.

Heinrich M, Rehm HJ. Formation of gluconic acid at low pH-values by free and immobilized Aspergillus niger during citric acid fermentation. Eur. J. Appl. Microbiol. Biotechnol. 15: 88-92, 1982.

Doelger WP, Prescott SC. Citric acid fermentation. Ind. Eng. Chem. 26: 1142-1149, 1934.

Betts RE, et al. Microbial transformations of antitumor compounds. I. Conversion of acronycine to 9-hydroxyacronycine by Cunninghamella echinulata. J. Med. Chem. 17: 599-602, 1974. PubMed: 4829940

Hang YD, Woodams EE. Apple pomace: a potential substrate for citric acid production by Aspergillus niger. Biotechnol. Lett. 6: 763-764, 1984.

Roukas T, Kotzekidon P. Production of citric acid from brewery wastes by surface fermentation using Aspergillus niger. J. Food Sci. 51: 225-228, 1986.

Hoffmann JJ, et al. Hydroxygrindelane derivatives by microbial transformation. Phytochemistry 27: 2125-2127, 1988.

Arfmann HA, et al. Microbial omega-hydroxylation of trans-nerolidol and structurally related sesquiterpenoids. Biocatalysis 2: 59-67, 1988.

Roukas T, Alichanidis E. Citric acid production from beet molasses by cell cycle of Aspergillus niger. J. Ind. Microbiol. 7: 71-74, 1991.

Hoffmann JJ, et al. Formation of grindelane dimers by microbial transformation. Phytochemistry 31: 3045-3049, 1992.

Ibrahim AR, Abul-Hajj YJ. Microbiological transformation of (+/-)-flavanone and (+/-)-isoflavanone. J. Nat. Prod. 53: 644-656, 1990. PubMed: 2213034

Miura S, et al. Prostaglandin chemistry -- IV. Microbiological kinetic resolution and asymmetric hydrolysis of 3,5-diacetoxycyclopent-1-ene. Tetrahedron 32: 1893-1898, 1976.

Kiel H, et al. Citric acid fermentation by Aspergillus niger on low sugar concentrations and cotton waste. Appl. Environ. Microbiol. 42: 1-4, 1981.

Martinez-Culebras PV, et al. Molecular characterization of the black Aspergillus isolates responsible for ochratoxin A contamination in grapes and wine in relation to taxonomy of Aspergillus section Nigri. Int. J. Food Microbiol. 132: 33-41, 2009. PubMed: 19401261

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation