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Clostridium acetobutylicum McCoy et al. emend. Keis et al. (ATCC® 824)

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Strain Designations: [CCRC 10639, CCUG 42182, DSM 792, IAM 19013, IFO 13948, JCM 1419, KCTC 1790, L.S. McClung 2291, LMG 5710, McCoy and McClung strain W, NCCB 29024, NCCB 84048, NCIMB 8052, VKM B-1787]  /  Type Strain: yes  /  Biosafety Level: 1

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Deposited As Granulobacter pectinovorum (Stormer) Beijerinck
Verified By Whole-genome Sequencing
Strain Designations [CCRC 10639, CCUG 42182, DSM 792, IAM 19013, IFO 13948, JCM 1419, KCTC 1790, L.S. McClung 2291, LMG 5710, McCoy and McClung strain W, NCCB 29024, NCCB 84048, NCIMB 8052, VKM B-1787]
Assay of 4-aminobenzoic acid p-aminobenzoic acid
Degrades xylan
Produces acetone
Produces acidolysin
Produces autobacteriocin
Produces butyl alcohol butanol
Produces ethyl alcohol ethanol
Produces restriction endonuclease Cac824I
Transformation host
Plant-derived foodstuff, cornmeal
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genome Sequenced Strain

Clostridium acetobutylicum ATCC 824, complete genome

Type Strain yes
Genome sequenced strain
Verification of identity
Name of Depositor ER Weyer
Chain of Custody ATCC <-- ER Weyer
Plant-derived foodstuff, cornmeal

Lee SF, et al. Xylanolytic activity of Clostridium acetobutylicum. Appl. Environ. Microbiol. 50: 1068-1076, 1985.

Soucaille P, Goma G. Acetonobutylic fermentation by Clostridium acetobutylicum ATCC 824: Autobacteriocin production, properties, and effects. Curr. Microbiol. 13: 163-169, 1986.

Croux C, et al. Purification and characterization of acidolysin, an acidic metalloprotease produced by Clostridium acetobutylicum ATCC 824. Appl. Environ. Microbiol. 56: 3634-3642, 1990. PubMed: 2082818

Validation list no. 51. Int. J. Syst. Bacteriol. 44: 852, 1994.

J. Infect. Dis. 56: 333-346, 1935.

Mermelstein LD, et al. Expression of cloned homologous fermentative genes in Clostridium acetobutylicum ATCC 824. Bio-Technology 10: 190-195, 1992. PubMed: 1368230

Ash C, et al. Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Antonie van Leeuwenhoek 64: 253-260, 1993. PubMed: 8085788

Wilkinson SR, et al. Phenotypic and genotypic differences between certain strains of Clostridium acetobutylicum. FEMS Microbiol. Lett. 125: 199-204, 1995.

Boynton ZL, et al. Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824. J. Bacteriol. 178: 3015-3024, 1996. PubMed: 8655474

Boynton ZL, et al. Cloning, sequencing, and expression of genes encoding phosphotransacetylase and acetate kinase from Clostridium acetobutlicum ATCC 824. Appl. Environ. Microbiol. 62: 2758-2766, 1996. PubMed: 8702268

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

Girdwood RH. The microbiological assay of vitamins and amino acids. J. Med. Lab. Technol. 20: 26-33, 1963. PubMed: 13948188

Lopez-Contreras AM, et al. Utilisation of saccharides in extruded domestic organic waste by Clostridium acetobutylicum ATCC 824 for production of acetone, butanol and ethanol. Appl. Microbiol. Biotechnol. 54: 162-167, 2000. PubMed: 10968627

Keis S, et al. Emended descriptions of Clostridium acetobutylicum and Clostridium beijerinckii, and descriptions of Clostridium saccharoperbutylacetonicum sp. nov. and Clostridium saccharobutylicum sp. nov.. Int. J. Syst. Evol. Microbiol. 51: 2095-2103, 2001. PubMed: 11760952

Bryant DL, Blaschek HP. Buffering as a means for increasing growth and butanol production by Clostridium acetobutylicum. J. Ind. Microbiol. 3: 49-55, 1988.

Cross References

Nucleotide (GenBank) : X78070 C. acetobutylicum (ATCC 824, T) 16S rRNA

Nucleotide (GenBank) : X70334 C. acetobutylicum gene for starch degrading enzyme

Nucleotide (GenBank) : M68865 Clostridium acetobutylicum autolytic lysozyme (lyc) gene, complete cds

Nucleotide (GenBank) : AF205034 Clostridium acetobutylicum sucrose utilization operon, complete sequence.

Nucleotide (GenBank) : M96945 Clostridium acetobutylicum butanol dehydrogenase isozyme A gene, complete cds

Nucleotide (GenBank) : M96946 Clostridium acetobutylicum butanol dehydrogenase isozyme B gene, complete cds

Nucleotide (GenBank) : AE001438 Clostridium acetobutylicum ATCC 824 megaplasmid pSOL1, complete sequence

Nucleotide (GenBank) : S77333 thl=thiolase {5' region} [Clostridium acetobutylicum, ATCC 824, Genomic, 81 nt]

Nucleotide (GenBank) : U16166 Clostridium acetobutylicum ATCC 824 16S ribosomal RNA (rrn) gene, partial sequence

Nucleotide (GenBank) : AF047822 Clostridium acetobutylicum catabolic acetolactate synthase (alsS) gene, complete cds

Nucleotide (GenBank) : U38234 Clostridium acetobutylicum phosphotransacetylase (pta) and acetate kinase (ack) genes, complete cds

Nucleotide (GenBank) : AF321779 Clostridium acetobutylicum plasmid pSOL1 aldehyde/alcohol dehydrogenase (adhE2) gene, complete cds

Nucleotide (GenBank) : L14744 Clostridium acetobutylicum phosphotransbutyrylase, complete cds; butyrate kinase, complete cds and ORF1

Nucleotide (GenBank) : L14817 Clostridium acetobutylicum transcriptional repressor (solR) and aldehyde-alcohol dehydrogenase (aad) genes, complete cds

Nucleotide (GenBank) : U52366 Clostridium acetobutylicum phosphofructokinase (pfk) gene, complete cds, and pyruvate kinase homolog (pyk) gene, partial cds

Nucleotide (GenBank) : M93363 Clostridium acetobutylicum acetoacetate decarboxylase, acetoacetyl-coenzyme A:acetate/butyrate:coenzyme A-transferase subunit, complete cds

Nucleotide (GenBank) : U08465 Clostridium acetobutylicum ATCC 824 acetyl coenzyme A acetyltransferase gene, complete cds, and homolog of B. subtilis orfX gene, partial cds

Nucleotide (GenBank) : U15277 Clostridium acetobutylicum putative regulatory protein and putative ortho phosphoribosyl transferase (pyrE) genes, partial cds and hydrogenase I (hydA) gene, complete cds

Nucleotide (GenBank) : U52368 Clostridium acetobutylicum rubredoxin oxidoreductase homolog and deformylase (def) genes, complete cds, and methionyl-tRNA formyltransferase homolog (fmt) gene, partial cds

Nucleotide (GenBank) : U07420 Clostridium acetobutylicum ATCC 824 putative sigma factor E (sigE) gene, putative sigma factor E processing enzyme (ORFA), gene and putative sigma factor G (sigG) gene, complete cds

Nucleotide (GenBank) : U52367 Clostridium acetobutylicum ATCC 824 F-type ATP synthase subunit a (atpA) gene, F-type ATP synthase subunit c (atpC) gene, and F-type ATP synthase subunit b (atpB) gene, complete cds

Nucleotide (GenBank) : U17110 Clostridium acetobutylicum crotonase (crt), putative butyryl-CoA dehydrogenase (BCD), putative a-subunit of electron-transfer flavoprotein (etfA), putative b-subunit of electron-transfer flavoprotein (etfB), and 3-hydroxybutyryl-CoA dehydrogenase

Nucleotide (GenBank) : AE001437 Clostridium acetobutylicum ATCC 824, complete genome

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
  1. Accessing genome sequencing data

    Date Updated: 9/30/2019

  2. Criteria for reference quality genomes

    Date Updated: 9/30/2019