Winkia neuii subsp. anitrata (Funke et al.) Nouioui et al.

1. Open vial.
2. Rehydrate the entire pellet with approximately 0.5 mL of #260 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #260 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
3. Use several drops of the primary broth tube to inoculate a #260 plate and/or #260 agar slant.
4. Incubate at 37°C for 24 hours in an atmosphere of 5% CO₂.

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