Streptomyces clavuligerus Higgens and Kastner (ATCC® 27064)

Strain Designations: [NRRL 3585]  /  Type Strain: yes  /  Biosafety Level: 1

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Strain Designations [NRRL 3585]
Application
Produces 5-(2-aminoadipyl)cysteinylvaline synthetase ACV synthetase
Produces cephalosporins
Produces clavulanic acid
Produces penicillin G benzylpenicillin
Produces penicillin N adicillin, cephalosporin N, isopenicillin N, synnematin B
Produces cephalosporin antibiotics 7-(5-amino-5-carboxyvaleramido)-3-carbamoyloxymethyl-3-cephem-4-carboxylic acid, and 7-(5-amino-5-carboxyvaleramido)-3-carbamoyloxymethyl -7-methoxy-3-cephem-4-carboxylic acid
Isolation
Soil, South America
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain yes
Comments
Parent of ATCC 700751
Medium ATCC® Medium 1877: ISP Medium 1
ATCC® Medium 196: Yeast malt extract agar
Growth Conditions
Temperature: 30°C
Atmosphere: Aerobic
Name of Depositor CE Higgens
U.S. Patent Number
Isolation
Soil, South America
References

Luengo JM, et al. Cyclization of phenylacetyl-L-cysteinyl-D-valine to benzylpenicillin using cell-free extracts of Streptomyces clavuligerus. J. Antibiot. 39: 1144-1147, 1986. PubMed: 3759664

Baldwin JE, et al. Isolation and partial characterization of ACV synthetase from Cephalosporium acremonium and Streptomyces clavuligerus. Evidence for the presence of phosphopantothenate in ACV synthetase. J. Antibiot. 44: 241-248, 1991. PubMed: 2010361

Zhang J, Demain AL. Regulation of ACV synthetase activity in the beta-lactam biosynthetic pathway of carbon sources and their metabolites. Arch. Microbiol. 158: 364-369, 1992.

Howarth TT, et al. Antibiotics. US Patent 4,525,352 dated Jun 25 1985

Perez-Llarena FJ, et al. A regulatory gene (ccaR) required for cephamycin and clavulanic acid production in Strptomyces clavuligerus: amplification results in overproduction of both beta-Lactam compounds. J. Bacteriol. 179: 2053-2059, 1997. PubMed: 9068654

Newton GL, et al. Distribution of thiols in microorganisms: mycothiol is a major thiol in most actinomycetes. J. Bacteriol. 178: 1990-1995, 1996. PubMed: 8606174

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

Mahro B, Demain AL. In vivo conversion of penicillin N into a cephalosporin type antibiotic by a non-producing mutant of Streptomyces clavuligerus. Appl. Microbiol. Biotechnol. 27: 272-275, 1987.

Higgens CE, Kastner RE. Streptomyces clavuligerus sp. nov., a beta-lactam antibiotic producer. Int. J. Syst. Bacteriol. 21: 326-331, 1971.

type strain

Cross References

Nucleotide (GenBank) : A38835 Sequence 2 from Patent WO9412654.

Nucleotide (GenBank) : A39664 Sequence 1 from Patent WO9418326.

Nucleotide (GenBank) : A42941 Sequence 2 from Patent WO9503416.

Nucleotide (GenBank) : AJ000671 Streptomyces clavuligerus car and claR genes.

Nucleotide (GenBank) : AJ001743 Streptomyces clavuligerus pcd, cmcT and pbp2 genes.

Nucleotide (GenBank) : Z81324 S.clavuligerus ccaR and blp genes and open open reading frame.

Nucleotide (GenBank) : AF038457 Streptomyces clavuligerus strain ATCC 27064 plasmid pSCL2 terminus.

Nucleotide (GenBank) : L06213 Streptomyces clavuligerus clavaminate synthase 1 (cs1) gene, complete cds.

Nucleotide (GenBank) : Z49111 Streptomyces clavuligerus arginine biosynthesis cluster (argCJBDRGH genes).

Nucleotide (GenBank) : AJ238211 Streptomyces clavuligerus ORF1, encoding a protein involved in clavulanic acid biosynthesis.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation