We offer you several custom solutions to quantitate microbial and viral strains including titering, qPCR, and droplet digital PCR. You may order these tests separately or as part of the quality control testing with the production of microbial banks.
Viral quantification is available to determine infectious virus particles in a sample. As appropriate, tissue culture infective dose (TCID50), chicken embryo infectious dose (CEID50), and plaque assays for measuring the number of plaque forming units (PFU) are methods used for viral titering at ATCC.
- TCID50 quantifies the amount of virus required to produce a cytopathic effect in 50% of inoculated culture cells
- CEID50 for viruses normally propagated in chicken eggs (such as influenza virus) viral titer is calculated as the chicken embryo infectious dose
- The viral plaque assay measures the ability of a single infectious virus to form a plaque on a cell culture monolayer. The number of plaque forming units (PFU) is determined per volume of inoculum
- Bacterial and fungal cultures can be titered via determining the number of colony forming unites per milliliter (CFU/mL)
- Quantitation of Chlamydia trachomatis strains at ATCC utilizes the IFU (inclusion forming unit) assay method to determine the titer. The IFU Assay determines the number of infectious units (elementary bodies) per preparation, by counting the number of inclusion bodies produced by elementary bodies in a dilution set
- Hemocytometers are commonly used to determine protist cell density, which is reported as the number of cell counts per volume, typically cells/mL
- qPCR and Droplet Digital PCR are quantitation methods used at ATCC for the quantitation of viral, bacterial, fungal, and protist strains as well as nucleic acids
- qPCR allows for the determination of the copy number of template DNA with accuracy and high sensitivity
- Quantification by genome copy number using Droplet Digital PCR provides an absolute count of target DNA copies per input sample without the need for running standard curves, making this technique ideal for measurements of target DNA, viral load analysis and microbial quantification.
- Offering flexible solutions, we can produce and titer from one to several vials in ready to use frozen formats or a format of your choice
- Pre- and/or post-freeze titers available
- Looking to outsource your COVID-19–related assays or materials? Let us know—our custom services team can help.
Material Inactivation Services
Infectious materials must be inactivated prior to use in assays or development work. Inactivation requires specialized technologies, skills, and suites. It is a risky process that requires a significant infrastructure (e.g., BSL level lab space, clean rooms, certifications, etc.). We are one of very few providers in the world to provide this service. Our services in this area include:
- Material inactivation (Bacteria/Virus)
- Formalin (bacteria), BPL (Virus)
- Other chemical treatments
- QC testing/sample analysis
- Viability assessment (reduction in agent titer)
- Titer methods
- Growth/no-growth methods
- Viral quantitative assays
- Antigenicity assessment (conservation of immunoreactivity)
- Conservation of reactivity in molecular assay
Deliverables: Inactivated materials as specified in the statement of work.
Contact us using the form below for more information on our services, or to let us know that you are ready to start your project.
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