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Viral Quantitation

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Our experts will quantify microbial strains for you

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We offer you several custom solutions to quantitate microbial and viral strains including titering, qPCR, and droplet digital PCR. You may order these tests separately or as part of the quality control testing with the production of microbial banks.

Viral quantification is available to determine infectious virus particles in a sample. As appropriate, tissue culture infective dose (TCID50), chicken embryo infectious dose (CEID50), and plaque assays  for measuring the number of plaque forming units (PFU) are methods used for viral titering at ATCC.  

Our offerings:

  • TCID50 quantifies the amount of virus required to produce a cytopathic effect in 50% of inoculated culture cells
  • CEID50 for viruses normally propagated in chicken eggs (such as influenza virus) viral titer is calculated as the chicken embryo infectious dose
  • The viral plaque assay measures the ability of a single infectious virus to form a plaque on a cell culture monolayer. The number of plaque forming units (PFU) is determined per volume of inoculum
  • Bacterial and fungal cultures can be titered via determining the number of colony forming unites per milliliter (CFU/mL)
  • Quantitation of Chlamydia trachomatis strains at ATCC utilizes the IFU (inclusion forming unit) assay method to determine the titer. The IFU Assay determines the number of infectious units (elementary bodies) per preparation, by counting the number of inclusion bodies produced by elementary bodies in a dilution set
  • Hemocytometers are commonly used to determine protist cell density, which is reported as the number of cell counts per volume, typically cells/mL
  • qPCR and Droplet Digital PCR are quantitation methods used at ATCC for the quantitation of viral, bacterial, fungal, and protist strains as well as nucleic acids
  • qPCR allows for the determination of the copy number of template DNA with accuracy and high sensitivity
  • Quantification by genome copy number using Droplet Digital PCR provides an absolute count of target DNA copies per input sample without the need for running standard curves, making this technique ideal for measurements of target DNA, viral load analysis and microbial quantification.
  • Offering flexible solutions, we can produce and titer from one to several vials in ready to use frozen formats or a format of your choice
  • Pre- and/or post-freeze titers available
  • Looking to outsource your COVID-19–related assays or materials? Let us know—our custom services team can help.

Material Inactivation Services

Infectious materials must be inactivated prior to use in assays or development work. Inactivation requires specialized technologies, skills, and suites. It is a risky process that requires a significant infrastructure (e.g., BSL level lab space, clean rooms, certifications, etc.). We are one of very few providers in the world to provide this service. Our services in this area include:

  • Material inactivation (Bacteria/Virus)
  • Formalin (bacteria), BPL (Virus)
  • Heat
  • Solvent/Detergent
  • Other chemical treatments
  • QC testing/sample analysis
  • Viability assessment (reduction in agent titer)
  • Titer methods
  • Growth/no-growth methods
  • Viral quantitative assays
  • Antigenicity assessment (conservation of immunoreactivity)
  • Conservation of reactivity in molecular assay

Deliverables: Inactivated materials as specified in the statement of work.

Blue fluorescent staining of Histoplasma capsulatum fungus.

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Custom quantitation

Contact us using the form below for more information on our services, or to let us know that you are ready to start your project.

Our laboratories are able to provide you with customized options grounded in decades of industry knowledge, making us your preferred strategic partner. Our team works with you through all stages of your custom project process, from the initial inquiry, design and initiation of your project, to the production, characterization, packaging, storage and distribution of your biological materials. This allows you to focus on your core competencies while we focus on what we do best, producing, managing and delivering the highest quality biological materials, globally.

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Cell Bank Production

We have been the authority on cell and microbial banking for nearly 100 years. Our laboratory and storage facilities are first-in-class for the large-capacity storage of cell lines, microorganisms, and other biological materials. We can support any cell banking need and will work with you at all stages of the cell bank development process from the initiation of your project to the large-scale production of your cells and microbes.

Blue and green metastatic carcinoma cells.

Cell Engineering

Cell engineering involves physical and engineering principles to control cell behavior. At ATCC, we utilize a number of cell engineering processes including hTERT immortalization and CRISPR/Cas9 gene editing for site-specific genome engineering of cell lines.

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Mycoplasma Testing

ATCC offers two options for testing for mycoplasma, the notoriously common bacteria that quickly infiltrate and destroy cell cultures: PCR-based mycoplasma testing or culture-based testing (Hoechst DNA staining and direct culture testing).

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Challenges and Solutions in the Development and Validation of Molecular-based Assays

Discover more about the challenges associated with evaluating the analytical performance of a molecular-based assay and find guidance on selecting the ideal reference materials.

Gloved hand using syringe for injection during egg inoculation in laboratory. Webinar

The Art and Science of Growing Animal Viruses in the Laboratory

Employing good aseptic technique and understanding the specific culture requirements of a strain are essential for optimal viral growth. In this webinar, we discuss the basic principles for propagating animal viruses in the laboratory, providing an in-depth look at viral culturing practices in both cell culture and embryonated chicken eggs.