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alcian blue stain airway histology primary bronchial cells

Does Differentiation Matter? Comparing the Toxicological Response Between Airway Epithelial Models Webinar

November 02, 2022, at 12:00 PM ET

Abstract

Traditional in vitro airway models are often unable to provide meaningful and accurate toxicological assessments of candidate compounds due to their lack of physiological relevance. However, advanced in vitro 3-D models may provide more predictive information for use in pharmaceutical development and basic research. To test this, ATCC scientists constructed 3-D airway models comprising fully differentiated or undifferentiated primary human bronchial tracheal epithelial cells (HBECs). They then challenged these in vitro models to short and long term exposure to the toxicant cadmium chloride and the antimicrobial pentamidine. Join this webinar to see data suggesting that these advanced models can be used to assess the toxicological response to both short- and long-term exposure to unknown compounds, serving as useful tools for future airway toxicity research. After the presentation, join us for a lively discussion as our experts answer your questions.

Key Points

  • This study demonstrates the utility of airway models as physiologically relevant models for safety research using compounds relevant to industrial hygiene and pharmaceutical development.
  • We compared toxicological response in airway models to 2-D models comprised of freshly seeded undifferentiated HBECs. Depending on the compound administered, significant differences in response were observed between the two different models.
  • This report also provides an overview on how to perform long-term testing on airway models.

Presenter

Kevin Tyo, headshot.
Kevin Tyo, PhD
Scientist, ATCC

Dr. Kevin Tyo is a Scientist in Research and Development at ATCC with over 10 years of experience in biological research. In his current role, Dr. Tyo develops and evaluates advanced in vitro co-culture models, as well as conducts toxicological testing. Dr. Tyo received his Ph.D. in Pharmacology and Toxicology from the University of Louisville in 2019, where he designed and tested topical drug delivery platforms that provided sustained release of antiviral therapeutics.

Toxicology Resources

Green and blue NuLi lung epithelial small airway cells.

hTERT-immortalized Cell Culture Guide

Follow these cell culture techniques to guarantee the viability of your cells for toxicity testing.

Get Best Practices
Fluorescent orange and black primary bronchial tracheal epithelial cells.

ATCC Human Bronchial/Tracheal Epithelial Cells

ATCC investigated the properties of primary HBECs and validated them as an in vitro model to improve respiratory studies.

Read the Application Note
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