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The Importance of Standardizing Lyophilization Techniques in Microbial Preservation

Rack of glass ampoules containing

ASM Microbe 2023

Houston, Texas, United States

June 17, 2023


Storage of microorganisms in a quantitative format is essential for modern industrial quality control and other related applications. Lyophilization is one of the most widely used techniques for preserving microorganisms in a desiccated format as it allows lab workers to avoid expensive ultra-cold chain storage and related distribution challenges. However, lyophilization is a complex process that involves phase changes of the extracellular environment, and the outcome is highly influenced by the compositional, thermal, and pressure changes of the materials. In this study, we evaluate how changes in lyophilization formulation affect microbial viability and protein expression characteristics using Escherichia coli (ATCC 8739) as a model. Our results highlight the need for optimization of lyophilization formulation for desiccated storage of microorganisms. Further studies are required to identify the standardized lyophilization processes.   

Download the poster to explore our data on the optimization of lyophilization formulation



Jyoti Jha, headshot

Jyoti K. Jha. PhD

Senior Scientist, BioNexus Cryobiology, ATCC

Dr. Jyoti K. Jha is a research and development scientist with over 15 years of experience in molecular biology and biochemistry of microbes. He previously worked at Elanco and Rise Therapeutics as a senior scientist for microbial product development. Currently, Dr. Jha develops and improves the mode of microbial preservation formulation and format for new products. Dr. Jha received a PhD from the University of Calcutta in Biotechnology and performed post-doctoral work at National Institutes of Health (NIH/NCI). Dr. Jha has published more than 25 peer reviewed papers as lead and/or co-author.