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Escherichia coli (Migula) Castellani and Chalmers


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Escherichia coli strain Crooks is a whole-genome sequenced bacterial strain that was isolated from feces. This product has applications as a quality control strain in testing antimicrobial handwashing formulations, media testing, efficacy testing, and bioresistance testing.
Product category
Enterobacteriaceae, Escherichia
Strain designation
Type strain
Genome sequenced strain
Isolation source
Food testing
Media testing
Quality control
Water testing
Pharmaceutical testing
Product format
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Assay of antimicrobial preservatives
Bioresistance testing
Efficacy testing
Media testing
Preparatory test control
Quality control strain
Testing antimicrobial handwashing formulations
Reduces dehydroascorbic acid
Food testing
Pharmaceutical and Personal Care
Quality control strain for Biosynth and Difco products
Recommended as a test microorganism for EP 2.6.13 Microbial Examination of Non-sterile Products: Test for Specified Micro-organisms
Recommended as a test microorganism for EP 2.6.31 Microbiological Examination of Herbal Medicinal Products for Oral Use
Recommended as a test microorganism for EP 5.1.3 Efficacy of Antimicrobial Preservation
Recommended as a test microorganism for JP 16 4.05 II Microbiological Examination of Non-sterile Products: Tests for Specified Micro-organisms
Recommended as a test microorganism for JP 16 5.02 Microbial Limit Test for Crude Drugs
Recommended as a test microorganism for JP 16 G4 Microorganisms: Preservatives-Effectiveness Tests
Recommended as a test microorganism for JP 16 G8 Quality Control of Water for Pharmaceutical Use
Recommended as a test microorganism for USP <2021> Microbial Enumeration Tests
Recommended as a test microorganism for USP <51> Antimicrobial Effectiveness Testing
Recommended as a test microorganism for USP <62> Microbiological Examination of Nonsterile Products: Tests for Specified Microorganisms

Recommended as a test microorganism for SNV SN 195924: Textile fabrics; determination of the antibacterial activity: germ count method

Recommended as a test microorganism for ASTM E640: Standard Test Method for Preservatives in Water-Containing Cosmetics

Recommended as a test microorganism for ISO 11930: Cosmetics — Microbiology — Evaluation of the antimicrobial protection of a cosmetic product

Recommended as a test microorganism for ISO 22196: Measurement of antibacterial activity on plastics and other non-porous surfaces

Recommended as a test microorganism for JIS Z 2801: Assessment of Antimicrobial Activity of Hard Non-Porous Surfaces



This strain has been sequenced at ATCC and does not contain the stx1 or stx2 genes for Shiga toxin production.
Toxin genes
stx1 (Shiga toxin 1) negative
stx2 (Shiga toxin 2) negative

Handling information

Handling procedure
  1. Open vial according to enclosed instructions.
  2. Using a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mLpipette. Rehydrate the entire pellet.
  3. Aseptically transfer this aliquot back into the broth tube. Mix well.
  4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate.
  5. Incubate the tubes and plate at 37°C for 24 hours.
Handling notes
This product is available as a certified reference material (ATCC CRM-8739).
Genomic DNA is available for this product (ATCC 8739D-5, 8739DQ).
Additional information on this culture is available on the ATCC web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Escherichia coli (Migula) Castellani and Chalmers
IC Gunsalus
Chain of custody
ATCC <-- IC Gunsalus <-- GC Crooks
Cross references
GenBank CP000946 Escherichia coli ATCC 8739, complete genome
GenBank 4002730 Escherichia coli C str. ATCC 8739 finished genome

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

ASTM International Standard Test Method for Preservatives in Water-Containing Cosmetics. West Conshohocken, PA

J. Biol. Chem. 141: 853, 1941.

British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI B, 2003

British Pharmacopoeia Commission Efficacy of antimicrobial preservation. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI C, 2003

European Pharmacopoeia Commission Microbial contamination of products not required to comply with the test for sterility (total viable aerobic count). Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.6.12, 1997

View All Curated Citations for this Product

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