Genetic Stability of Microorganisms During Preservation and Propagation
Poster
SIMB Annual Meeting 2023
Minneapolis, Minnesota, United States
July 30, 2023
Abstract
Post-preservation propagation of
microorganisms is known to create genetic variability that might adversely affect the quality of downstream applications like quantitative analytical assays. In this study, we investigated the genetic stability of microorganisms under different passage conditions following long-term stabilization via lyophilization. Here, wild-type
Escherichia coli (
ATCC 8739) was used as a model microorganism, and next-generation sequencing was used to evaluate genetic variability. From our analysis, we observed that lyophilization did not significantly impact genetic stability while an increased number of passages did. These findings underscore the need to restrict the use of model microorganisms to low-passage numbers.
Download the poster to discover how preservation and propagation affect microbial genetic stability
Download
Jyoti K Jha, PhD
Senior Scientist, BioNexus Cryobiology, ATCC
Dr. Jyoti K. Jha is a research and development scientist with over 15 years of experience in molecular biology and biochemistry of microbes. He previously worked at Elanco and Rise Therapeutics as a senior scientist for microbial product development. Currently, Dr. Jha develops and improves the mode of microbial preservation formulation and format for new products. Dr. Jha received a PhD from the University of Calcutta in Biotechnology and performed post-doctoral work at National Institutes of Health (NIH/NCI). Dr. Jha has published more than 25 peer reviewed papers as lead and/or co-author.
Explore our featured resources
TSB with 10% Glycerol
Trypticase soy broth (TSB) with 10% glycerol (v/v) is used as a freeze medium for storage of non-fastidious bacteria, fungi, and yeast.
order today
CoolCell LX Alcohol-Free Cryopreservation Container
The CoolCell LX container, with a -80°C freezer or dry ice locker, will provide the freezing rate of -1°C per minute that’s ideal for cryopreservation.
order now
Bacterial Culture Guide
Explore technical information for the growth, propagation, preservation, and application of fastidious and non-fastidious bacterial species.
Learn More
Mycology Culture Guide
Detailed information on the growth, handling, propagation, preservation, and application of yeasts and filamentous fungi.
Get the Guide
Protistology Culture Guide
Tips and techniques for the growth, propagation, preservation, and safe handling of protists.
Learn More
Virology Culture Guide
Explore tips and techniques for propagating viruses in tissue culture and embryonated chicken eggs.
Explore the Guide