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Genetic Stability of Microorganisms During Preservation and Propagation

Gloved hand using tweezers to hold frozen vial above open liquid nitrogen cryopreservation tank.

SIMB Annual Meeting 2023

Minneapolis, Minnesota, United States

July 30, 2023


Post-preservation propagation of microorganisms is known to create genetic variability that might adversely affect the quality of downstream applications like quantitative analytical assays. In this study, we investigated the genetic stability of microorganisms under different passage conditions following long-term stabilization via lyophilization. Here, wild-type Escherichia coli (ATCC 8739) was used as a model microorganism, and next-generation sequencing was used to evaluate genetic variability. From our analysis, we observed that lyophilization did not significantly impact genetic stability while an increased number of passages did. These findings underscore the need to restrict the use of model microorganisms to low-passage numbers. 

Download the poster to discover how preservation and propagation affect microbial genetic stability


Jyoti Jha, headshot

Jyoti K. Jha. PhD

Senior Scientist, BioNexus Cryobiology, ATCC

Dr. Jyoti K. Jha is a research and development scientist with over 15 years of experience in molecular biology and biochemistry of microbes. He previously worked at Elanco and Rise Therapeutics as a senior scientist for microbial product development. Currently, Dr. Jha develops and improves the mode of microbial preservation formulation and format for new products. Dr. Jha received a PhD from the University of Calcutta in Biotechnology and performed post-doctoral work at National Institutes of Health (NIH/NCI). Dr. Jha has published more than 25 peer reviewed papers as lead and/or co-author.