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Chlamydia trachomatis

VR-571B

Chlamydia trachomatis Trachoma type A strain HAR-13 [strain Har 13] is propagated in McCoy [McCoy B] cells (ATCC CRL-1696). This strain was isolated from the conjunctiva of a 4-year-old child with trachoma and was deposited by the Centers for Disease Control and Prevention.
Product category
Bacteria
Classification
Chlamydiaceae, Chlamydia
Strain designation
Trachoma type A strain HAR-13 [strain Har 13]
Type strain
Yes
Isolation source
Conjunctiva from a child with trachoma, human conjunctiva.
Product format
Frozen
Storage conditions
-70°C or colder
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Documentation

Biosafety Icon Biosafety Level 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

Characteristics

Serotype
A
Comments
This preparation has tested positive for Mycoplasma contamination.
Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC CRL-1696) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus.
Mycoplasma contamination
Not detected

Handling information

Host
McCoy [McCoy B] (ATCC CRL-1696)
Host Range: McCoy cells (ATCC CRL-1696) Chicken embryo, yolk sac; anti-metabolite treated L-929 cells (ATCC CCL-1); HeLa 229 cells (ATCC CCL-2.1)
Effects
CPE; cytoplasmic inclusions
Complete medium
DMEM (ATCC 30-2002) + 10% prescreened FBS + 10 mM HEPES + 2 µg/mL Cycloheximide (Sigma C-4859 Ready-Made)
Temperature
36°C
Atmosphere
95% Air, 5% CO2
Incubation
3 days, a 5% CO2 in air atmosphere is recommended
Recommendation for infection
For best results, cells should be 24 to 48 hours old and 90-100% confluent
Handling notes
Note that activities with high potential for aerosol production require BSL 3 facilities and practices. This preparation has tested positive for mycoplasma contamination. Suggested protocol for propagation: Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour. Feed with fresh growth medium containing FBS prescreened or pretested against having Chlamydia antibodies and 2µg/mL cycloheximide. Incubate at 36°C for 3 days.
Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC CRL-1696) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus.
Key abbreviations
°C, Degrees Celsius
CO2, Carbon dioxide
CDC, Centers for Disease Control and Prevention
DMEM, Dulbecco's Modified Eagle's Medium
FBS, Fetal bovine serum
HeLa, Human cervical carcinoma cells
HEPES, N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
TCID[50], Median tissue culture infective dose

History

Deposited as
Trachoma serotype a
Depositors
CDC
Type of isolate
Human
Patient age
4 years

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Murray ES. . Am. J. Trop. Med. Hyg. 29: 116, 1980.

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

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