VR-3342HK

VR-3342HK ^™

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This product is a preparation of Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) strain USA/MD-HP05285/2021 (Delta variant) that has been inactivated by heating at 65°C for 30 minutes and is therefore unable to replicate. This heat-inactivated preparation is whole-genome sequenced and can be used as a process control in the development of SARS-CoV-2 molecular diagnostic tools and can also be used to make a dilution series to estimate the level of detection.

Due to the dynamic nature of lineage determination using PANGO, it is recommended to confirm the provided GISAID information prior to initiation of work.

Product category: Viruses
Product type: Animal virus

Inactivated organism
Strain designation: USA/MD-HP05285/2021
Genome sequenced strain: Yes
Isolation source: Human, April 26, 2021
Geographical isolation: United States; Maryland
Applications: Assay development

Infectious disease research

Respiratory disease research

Zoonotic disease research
Product format: Frozen
Shipping information: Each vial contains approximately 0.25 mL of heat-inactivated, clarified cell lysate and supernatant from Calu-3 cells (ATCC HTB-55) infected with SARS-CoV-2 strain USA/MD-HP05285/2021. The vial should be centrifuged prior to opening.
Storage conditions: -70°C or colder

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Price: $1,285.00 ea

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Documentation

Product sheet

BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

General

Specific applications: Assay development
Assay control
Research
This product is intended for use in molecular assays or as a process control (e.g., viral RNA extraction through qPCR amplification process). ATCC VR-3342HK can also be used to make a dilution series to estimate level of detection.

Characteristics

Comments

This isolate is lineage AY.24 (Pango v.3.1.14 2021-09-28), Delta (B.1.617.2-like) (Scorpio), and GISIAD clade GK using Phylogenetic Assignment of Named Global Outbreak lineages (PANGO) tool. Due to the dynamic nature of lineage determination using PANGO, discrepancies may exist between vial labels and the current lineage assignment.

The complete genome of the clinical isolate of SARS-CoV-2, hCoV-19/USA/MD-HP05285/2021 has been sequenced (GISAID: EPI_ISL_2103264). The following mutations are present in the clinical isolate: Spike D614G, Spike D950N, Spike L452R, Spike P681R, Spike T19R, Spike T478K, M (Membrane protein) I82T, N (Nucleocapsid) D63G, N D377Y, NS3 S26L, NSP3 (Non-structural protein 3) P822L, NSP4 (Non-structural protein 4) A446V, NSP6 (Non-structural protein 6) T181I, NSP6 V149A, NSP10 (Non-structural protein 10) L133F, NSP12 (Non-structural protein 12) G671S, NSP12 P323L, NSP13 (Non-structural protein 13) P77L. It was labelled as VOC Delta GK/478K.V1 (B.1.617.2+AY.x), first detected in India by the World Health Organization (WHO).

This product is a preparation of Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) strain USA/MD-HP05285/2021 that has been inactivated by heating at 65°C for 30 minutes and is therefore unable to replicate.

The long-term stability of this preparation is not known at this time. It is recommended that users confirm the activity of the product if not used within three months of receipt.

Handling information

Handling notes

Due to high demand, this product is limited to 20 per organization per 60 days.

This product is a preparation of Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) strain USA/MD-HP05285/2021 that has been inactivated by heating at 65° for 30 minutes and is therefore unable to replicate.

The long-term stability of this preparation is not known at this time. It is recommended that users confirm the activity of the product if not used within three months of receipt.

Quality control specifications

Volume: 250 μL
Verification method: Whole-genome Sequencing

History

Depositors: Centers for Disease Control and Prevention (CDC)
Type of isolate: Human
Year of origin: 2021

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Baczenas JJ, et al. Propagation of SARS-CoV-2 in Calu-3 Cells to Eliminate Mutations in the Furin Cleavage Site of Spike. Viruses 13(12): 2434, 2021.

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