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Quantitative Synthetic Monkeypox virus DNA

VR-3270SD

ATCC Quantitative Synthetic Monkeypox virus DNA can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load. Preparation includes fragments from J2L, D14L, F3L, F8L, A27L, A29L, B6R, B7R, and N3R regions. The sequence of this research use only (RUO) construct was designed based on several published assays.

Product category
Viruses
Product type
Molecular standard
Nucleic acid
Organism
Monkeypox virus, MPXV
Applications
Infectious disease research
Assay development
Zoonotic disease research
Specification range
≥ 1 x 105 to 1 x 106 copies/μL
Volume
100 μL
Product format
Frozen
Shipping information

Shipped in a proprietary stabilization matrix

Storage conditions
-20°C or colder
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Price: $541.00 ea
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information

General

Specific applications
ATCC Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load.

Characteristics

Genetic target
Preparation includes fragments from J2L, D14L, F3L, F8L, A27L, A29L, B6R, B7R, and N3R regions.
Comments

The sequence of this research use only (RUO) construct was designed based on several published assays.

Manufactured under ISO 13485 guidance

The recipient testing laboratory is responsible for generating validation or verification data as applicable to establish performance characteristics as required by the testing laboratory’s policies, applicable regulations, and quality system standards.

Handling information

Handling procedure
  1. Thaw the vial on ice. Avoid exposing the synthetic DNA to repeated freeze-thaw cycles as it may result in degradation of the DNA and variation in copy number.
  2. Gently mix the sample to ensure an even distribution of material.
  3. Briefly centrifuge the tube before opening to ensure all liquid is at the bottom.
Handling notes
Aliquoting is highly recommended to avoid multiple freeze-thaws, which can damage the synthetic DNA. This construct is synthetically derived and therefore does not contain any viable material and cannot replicate.

The following primers and probe can be used with this nucleic acid preparation.
Forward primer (5’ to 3’): CATCTATTATAGCATCAGCATCAGA
Reverse primer (5’ to 3’): GATACTCCTCCTCGTTGGTCTAC
Probe (5’ to 3’): JOE/TGTAGGCCGTGTATCAGCATCCATT/BHQ1

Quality control specifications

Quality accreditation
Manufactured under ISO 13485 guidance

History

Depositors
ATCC

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The synthetically engineered sequence of the product constitutes intellectual property belonging to ATCC. Unauthorized use, including sequencing, modification, or reverse-engineering, of the product is expressly prohibited without prior ATCC consent.

Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

References

Curated Citations

Li Y, et al. Detection of monkeypox virus with real-time PCR assays. J Clin Virol 36(3): 194-203, 2006. PubMed: 16731033

RJ Grant, et al. Application of the Ibis-T5000 pan-Orthopoxvirus assay to quantitatively detect monkeypox viral loads in clinical specimens from macaques experimentally infected with aerosolized monkeypox virus. Am J Trop Med Hyg. 82(2): 318-323, 2010. PubMed: 20134011

CL Hutson, et al. A prairie dog animal model of systemic orthopoxvirus disease using West African and Congo Basin strains of monkeypox virus. J Gen Virol. 90(Pt 2): 323-333, 2009. PubMed: 19141441

CL Hutson, et al. Comparison of West African and Congo Basin monkeypox viruses in BALB/c and C57BL/6 mice. PLoS One. 5(1):e8912 doi: 10.1371/journal.pone.0008912, 2010. PubMed: 20111702

SN Shchelkunov, et al. Species-specific identification of variola, monkeypox, cowpox, and vaccinia viruses by multiplex real-time PCR assay. J Virol Methods. 175(2): 163-169, 2011. PubMed: 21635922

View All Curated Citations for this Product

Frequently Asked Questions

Need assistance with this product? Contact our Technical Support team.

Telephone

Telephone

US and Puerto Rico

800-638-6597

Outside the US

+1-703-365-2700
hours

Hours of Operation

Monday-Friday
9:30am - 5:30pm
US Eastern Time