VR-1947

VR-1947 ^™

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Human astrovirus strain Type 5 is propagated in Caco-2 [Caco2] cells (ATCC HTB-37). This strain was isolated from a human stool sample in Oxford, United Kingdom, and was deposited by the Centers for Disease Control and Prevention, Division of Viral Diseases. It can be used in a variety of applications including virucide testing, antiviral studies, spiking studies, enteric disease research, assay development, and vaccine development.

Product category: Viruses
Product type: Animal virus
Strain designation: Type 5
Genome sequenced strain: Yes
Isolation source: Human stool sample
Geographical isolation: United Kingdom; Oxford
Applications: Infectious disease research

Assay development

Vaccine development

Enteric disease research
Product format: Frozen
Storage conditions: -70°C or colder

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Documentation

Product sheet

BSL 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Caco-2 [Caco2]

HTB-37

Eagle's Minimum Essential Medium (EMEM)

30-2003

Dulbecco's Phosphate Buffered Saline (D-PBS), 1X

30-2200

General

Specific applications: Virucide testing
In vitro infectivity assay
Antiviral Studies
Spiking studies
ELISpot Assay
Virus neutralization assay
Virus ultrastructural studies
Immunological-based assays
PCR-based assay validation
Research and development
Assay development
Vaccine development

Characteristics

Comments: Freeze-thaw twice at harvest.

Handling information

Host: Caco-2 [Caco2] (ATCC HTB-37)
Effects: CPE may not be visible – confirm infection by IFA; sloughing and detachment may result from trypsin supplemented growth medium
Temperature: 37°C
Atmosphere: 95% Air, 5% CO₂
Incubation: 1-5 days at 37°C in a humidified 5% CO₂ atmosphere, until CPE is progressed through 80% of the monolayer.
Recommendation for infection: Plate cells 18-24 hours prior to infection and infect when cultures are 90-100% confluent. Pre-treat inoculum in EMEM (ATCC 30-2003) + 5 µg/mL 1X-S trypsin (Sigma-Aldrich^® T0303) for 30 minutes at 37°C. Remove medium and wash cells two times with DPBS. Inoculate with a small volume of virus (e.g. 1 mL per 25 cm²) diluted to provide an optimal MOI (e.g. 1.0). Adsorb 1 hour at 37°C in a humidified 5% CO₂ atmosphere. End adsorption by removing inoculum and adding virus growth medium.
Handling notes: Freeze-thaw twice at harvest.
Key abbreviations: °C, Degrees Celsius

CO₂, Carbon dioxide

D-PBS, Dulbecco's Phosphate-buffered saline

EMEM, Eagle's Minimum Essential Medium

MOI, Multiplicity of infection

Quality control specifications

Volume: 1.0 mL
Verification method: Whole-genome Sequencing

History

Deposited as: Human astrovirus
Depositors: J Vinje, CDC, Division of Viral Diseases
Type of isolate: Human

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Lee TW, Kurtz JB. Prevalence of human astrovirus serotypes in the Oxford region 1976-92, with evidence for two new serotypes. Epidemiol Infect. 1112(1): 187-193, 1994. PubMed: 8119358

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