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Murine norovirus 1

VR-1937

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Murine norovirus 1 (MNV-1) strain CW1 is propagated in RAW 264.7 cells (ATCC TIB-71). This strain was purified from an infected IFNαβγR-/- mouse brain homogenate. This animal virus has been verified by whole-genome sequencing and has has applications in enteric and infectious disease research.
Product category
Viruses
Product type
Animal virus
Classification
Caliciviridae, Norovirus
Strain designation
CW1
Common name
MNV-1
Genome sequenced strain
Yes
Isolation source
Purified from an infected IFNαβγR-/- mouse brain homogenate
Applications
Infectious disease research
Enteric disease research
Product format
Frozen
Storage conditions
-70°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

RAW 264.7

TIB-71

Detailed product information

Characteristics

Comments

The ATCC TIB-71 cell line is a macrophage transformed by Abelson murine leukemia virus and has been demonstrated to express ecotropic and polytropic MuLV, which may be detected by NGS. Additionally, a less than 2 kb fragment of Staphylococcus chromosome may be observed; attributable to the vector.

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.

Handling information

Host
RAW 264.7 (ATCC TIB-71)
Effects
cell sloughing; cell degeneration
Complete medium
EMEM (ATCC 30-2003) + 2% FBS (ATCC 30-2020)
Temperature
37°C
Atmosphere
95% Air, 5% CO2
Incubation
4-6 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 95% of the monolayer.
Recommendation for infection
Plate cells 18-24 hours prior to infection and infect when cultures are 60-70% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Handling notes

The ATCC TIB-71 cell line is a macrophage transformed by Abelson murine leukemia virus and has been demonstrated to express ecotropic and polytropic MuLV, which may be detected by NGS. Additionally, a less than 2 kb fragment of Staphylococcus chromosome may be observed; attributable to the vector.

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.

Key abbreviations
°C, Degrees Celsius
CO2, Carbon dioxide
EMEM, Eagle's Minimum Essential Medium
FBS, Fetal bovine serum
MOI, Multiplicity of infection
MuLV, Murine leukemia virus
NGS, next generation sequencing

Quality control specifications

Volume
1.0 mL
Verification method
Whole-genome Sequencing

History

Deposited as
Murine norovirus 1 (MNV-1) animal: CW1
Depositors
HW Virgin, Washington University
Type of isolate
Animal

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

For-profit Research Use License from Washington University, St. Louis

For-profit Organizations: For every order of this item, you must work directly with the contributor, Washington University in St. Louis to (i) negotiate a research-use license, and/or (ii) have the contributor provide authorization to ATCC to ship this item under your existing license. We cannot ship this item until we receive communication directly from Washington University in St. Louis that we are authorized to ship each order.

We are providing the following contact information, but this information may change without notice:
Washington University
Office of Technology Management
Attn. Associate Director Phone: 314-747-0920
Email: [email protected]

Once ATCC has received authorization from Washington University in St. Louis, your order will be reviewed, and this item will be released for shipment if all requirements are met. If you need assistance with your order, please contact our Customer Care team or your applicable distributor.

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

US Patent 7,041,444, Murine Calicivirus

US Patent 7,264,923, Norovirus Infected Cell Cultures and Uses Therefor

Hartley JW, et al. Expression of infectious murine leukemia viruses by RAW264.7 cells, a potential complication for studies with a widely used mouse macrophage cell line. Retrovirology. 4: 5:1, 2008. PubMed 18177500.

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