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Fibroblast Basal Medium

PCS-201-030

Fibroblast Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system. This system is designed to support fibroblasts derived from normal human epidermis. Fibroblast Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts.

  1. To support the proliferation and plating efficiency of fibroblasts (e.g., Dermal Fibroblasts, Normal, Human Neonatal [ATCC PCS-201-010], Dermal Fibroblasts, Normal, Human Neonatal, Mitomycin C Treated [ATCC PCS-201-010]), Fibroblast Basal Medium must be supplemented with one of the following kits:
    1. Fibroblast Growth Kit–Serum-Free (ATCC PCS-201-040)
    2. Fibroblast Growth Kit–Low Serum (ATCC PCS-201-041)
  2. Optional media supplements:
    1. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
    2. Phenol Red (ATCC PCS-999-001)
Product category
Media
Applications
Cell growth and viability
Volume
480 ml
Storage conditions
2°C to 8°C
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Documentation

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Detailed product information

General

Specific applications

Fibroblast Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC® Primary Cell Solutions™ system. This system is designed to support fibroblasts derived from normal human epidermis. Fibroblast Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts.

  1. To support the proliferation and plating efficiency of fibroblasts (e.g., Dermal Fibroblasts, Normal, Human Neonatal [ATCC PCS-201-010], Dermal Fibroblasts, Normal, Human Neonatal, Mitomycin C Treated [ATCC PCS-201-010]), Fibroblast Basal Medium must be supplemented with one of the following kits:
    1. Fibroblast Growth Kit–Serum-Free (ATCC PCS-201-040)
    2. Fibroblast Growth Kit–Low Serum (ATCC PCS-201-041)
  2. Optional media supplements:
    1. Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002)
    2. Phenol Red (ATCC PCS-999-001)

Handling information

Handling procedure
  1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
  2. Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates, prior to adding to the basal medium.
  3. Obtain one bottle of Fibroblast Basal Medium (480 mL) from cold storage.
  4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
  5. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the volume of each growth kit component, as indicated in Table 1 or 2, to the bottle of basal medium using a separate sterile pipette for each transfer.

Table 1. If using the Fibroblast Growth Kit–Serum-Free (ATCC® PCS-201-040), add the indicated volume for each component in the order shown.

Component

Volume

Final Concentration

L-glutamine

18.75 mL

7.5 mM

Hydrocortisone Hemisuccinate

0.5 mL

1 mg/mL

HLL Supplement

1.25 mL

HSA 500 mg/mL

Linoleic Acid 0.6 mM

Lecithin 0.6 mg/mL

rh FGF b

0.5 mL

5 ng/mL

rh EGF / TGF b-1 Supplement

0.5 mL

5 ng/mL

30 pg/mL

rh Insulin

0.5 mL

5 mg/mL

Ascorbic acid

0.5 mL

50 µg/mL

        

        Table 2. If using the Fibroblast Growth Kit–Low Serum (ATCC® PCS-201-041), add the indicated volume for each of the following components:

Component

Volume

Final Concentration

rh FGF b

0.5 mL

5 ng/mL

L-glutamine

18.75 mL

7.5 mM

Ascorbic acid

0.5 mL

50 mg/mL

Hydrocortisone Hemisuccinate

0.5 mL

1 mg/mL

rh Insulin

0.5 mL

5 mg/mL

Fetal Bovine Serum

10.0 mL

2%

 

Antimicrobials and phenol red are not required for proliferation but may be added if desired. The recommended volume of each optional component to be added to the complete growth media is summarized in Table 3.

 

Table 3. Addition of Antimicrobials/Antimycotics and Phenol Red (Optional)

Component

Volume

Final Concentration

Gentamicin-Amphotericin B Solution

0.5 mL

Gentamicin: 10 µg/mL

Amphotericin B: 0.25 µg/mL

Penicillin-Streptomycin-Amphotericin B Solution

0.5 mL

Penicillin: 10 Units/mL

Streptomycin: 10 µg/mL

Amphotericin B: 25 ng/mL

Phenol Red

0.5 mL

33 µM

 

  1. Tightly cap the bottle of complete growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the bottle.
  2. Complete growth media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, complete media is stable for 30 days. 
Cryopreservation
1. Check all containers for leakage or breakage.

2. Store the basal medium at 2°C to 8°C. Do not freeze. Protect from light. Do not exceed 37°C when warming the media prior to use. When stored in the dark at 2°C to 8°C, the product is stable until the expiration date on the label

Quality control specifications

Mycoplasma contamination
Not detected
Endotoxin
< 0.5 EU/mL
Osmolality
270 ± 10 mOsm/kg
pH
7.4 ± 0.2
Functional tests
Rate of proliferation and morphology.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

References

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