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Endothelial Cell Growth Kit-VEGF

PCS-100-041

Endothelial Cell Growth Kit-BBE (ATCC® PCS-100-040) and Endothelial Cell Growth Kit-VEGF (ATCC® PCS-100-041) each contain components that when added to Vascular Cell Basal Medium (ATCC® PCS-100-030) create a complete ATCC® Primary Cell Solution™ culture environment for endothelial cells derived from normal human large vessels (e.g., Normal Primary Human Umbilical Vein Endothelial Cells (HUVEC), ATCC® PCS-100-010 or Primary Aortic Endothelial Cells, ATCC® PCS-100-011). Your experimental design will dictate which Endothelial Cell Growth Kit should be used. Use of the Endothelial Cell Growth Kit-VEGF (ATCC® PCS-100-041) will support a faster rate of proliferation because of the presence of several purified human recombinant (rh) growth factors (rh VEGF, rh EGF, rh FGF basic and rh IGF-1) combined with heparin and hydrocortisone. Use of the Endothelial Cell Growth Kit-BBE (ATCC® PCS-100-040), which contains Bovine Brain Extract (BBE), is recommended if a less defined cell culture medium is desired.

Product category
Reagents
Comments
The Endothelial Cell Growth Kit-VEGF contains components that when added to Vascular Cell Basal Medium (ATCC PCS-100-030) create a complete, low serum culture environment for human large vessel endothelial cells. The final concentration for each component in complete endothelial growth medium is as follows:
  • rh VEGF: 5 ng/mL
  • rh EGF: 5 ng/mL
  • rh FGF basic: 5 ng/mL
  • rh IGF-1: 15 ng/mL
  • L-glutamine: 10 mM
  • Heparin sulfate: 0.75 Units/mL
  • Hydrocortisone: 1 µg/mL
  • Ascorbic acid: 50 µg/mL
  • Fetal bovine serum: 2%
Applications
Cell growth and viability
Product format
Frozen
Shipping information
1 kit
Storage conditions
-20°C or colder, -70°C for long-term storage
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Documentation

Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Primary Coronary Artery Endothelial Cells; Normal, Human (HCAEC)

PCS-100-020

Price: $761.00 ea

Primary Pulmonary Artery Endothelial Cells; Normal, Human (HPAEC)

PCS-100-022

Price: $714.00 ea

Primary Umbilical Vein Endothelial Cells; Normal, Human (HUVEC)

PCS-100-010

Price: $285.00 ea

Primary Aortic Endothelial Cells; Normal, Human (HAEC)

PCS-100-011

Price: $703.00 ea

Vascular Cell Basal Medium

PCS-100-030

Price: $55.00 ea

Endothelial Cell Growth Kit-VEGF

PCS-100-041

Price: $97.00 ea

Dulbecco's Phosphate Buffered Saline (D-PBS), 1X

30-2200

Price: $16.00 ea

Trypsin-EDTA for Primary Cells

PCS-999-003

Price: $28.00 ea

Trypsin Neutralizing Solution

PCS-999-004

Price: $28.00 ea

Penicillin-Streptomycin-Amphotericin B Solution

PCS-999-002

Price: $28.00 ea

Phenol Red

PCS-999-001

Price: $28.00 ea

Detailed product information

Handling information

Handling procedure

Unpacking and Storage Instructions

  1. Check all containers for leakage or breakage.
  2. Store the basal medium at 2°C to 8°C and the growth kit(s) at either -20°C in a freezer that is not self-defrosting or at -70°C for long-term storage. If thawed upon arrival, the growth kit should be stored at 2°C to 8°C and added to the basal medium within 72 hours of receipt.

Preparation of Complete Growth Media

  1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
  2. Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates, prior to adding to the basal medium.
  3. Obtain one bottle of Vascular Cell Basal Medium (475 mL) from cold storage.
  4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
  5. Using aseptic technique, and working in a laminar flow hood or biosafety cabinet transfer the volume of each growth kit component, as indicated in Table 1 or 2, to the bottle of basal medium using a separate sterile pipette for each transfer.
  6. Tightly cap the bottle of complete growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the bottle.
Complete media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, complete media is stable for 30 days.

Table 1. If using the Endothelial Cell Growth Kit-BBE (ATCC PCS-100-040), add the indicated volume for each component: 

Component

Volume

Final Concentration

Bovine Brain Extract (BBE)

1.0 mL

0.2%

rh EGF

0.5 mL

5 ng/mL

L-glutamine

25.0 mL

10 mM

Heparin sulfate

0.5 mL

0.75 Units/mL

Hydrocortisone hemisuccinate

0.5 mL

1 µg/mL

Fetal Bovine Serum

10.0 mL

2%

Ascorbic acid

0.5 mL

50 µg/mL

 

Table 2. If using the Endothelial Cell Growth Kit-VEGF (ATCC PCS-100-041), add the indicated volume for each component:

Component

Volume

Final Concentration

rh VEGF

0.5 mL

5 ng/mL

rh EGF

0.5 mL

5 ng/mL

rh FGF basic

0.5 mL

5 ng/mL

rh IGF-1

0.5 mL

15 ng/mL

L-glutamine

25.0 mL

10 mM

Heparin sulfate

0.5 mL

0.75 Units/mL

Hydrocortisone hemisuccinate

0.5 mL

1 µg/mL

Fetal Bovine Serum

10.0 mL

2%

Ascorbic acid

0.5 mL

50 µg/mL

Antimicrobials and phenol red are not required for proliferation but may be added if desired. The recommended volume of each optional component to be added to the complete growth media is summarized in Table 3.

Table 3. Addition of Antimicrobials/Antimycotics and Phenol Red (Optional)

Component

Volume

Final Concentration

Gentamicin-Amphotericin B Solution

0.5 mL

Gentamicin: 10 µg/mL

Amphotericin B: 0.25 µg/mL

Penicillin-Streptomycin-Amphotericin B Solution

0.5 mL

Penicillin: 10 Units/mL

Streptomycin: 10 µg/mL

Amphotericin B: 25 ng/mL

Phenol Red

0.5 mL

33 µM

 

Cryopreservation
1. Check all containers for leakage or breakage.
2. Store the basal medium at 2°C to 8°C and the growth kit(s) at either -20°C in a freezer that is not self-defrosting or at -70°C for long-term storage. If thawed upon arrival, the growth kit should be stored at 2°C to 8°C and added to the basal medium within 72 hours of receipt.

Quality control specifications

Bacterial and fungal testing
Not detected
Mycoplasma contamination
Not detected
Functional tests
Rate of proliferation and morphology

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

References

Frequently Asked Questions

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