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Rhizopus oryzae Went et Prinsen Geerligs


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An ampoule containing viable cells (yeast cells, spores, or agar cubes with mycelia) suspended in cryoprotectant.       

Product category
Strain designation
USDB 0224 [CBS 515.94, NRRL 22564]
Type strain
Genome sequenced strain
Isolation source
Fermented food
Geographical isolation
Biofuel production
Food production research
Product format
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
produces aspartic proteinases aspartyl proteinases
produces carboxyl proteinase

Potential biofuel production agent: lignocellulosic ethanol

Potential strain for mycoprotein production



sap1 sap2 sap3 sap4
After 6 days at 25°C colonies growing rapidly, floccose with white aerial mycelium.  Sporangiophores.straight or curved; sometimes swollen or branched; wall smooth or slightly rough; pale brown.  Sporangia globose or subglobose; wall spinous; black maturity.  Columellae.globose, subglobose or oval; wall usually smooth; pale brown.  Sporangiospores.elliptical, globose or polygonal; striated; 5-8 µm in length. Chlamydospores.abundant; globose (10-24 µm in diameter), elliptical, lemonshaped or cylindrical.  Rhizoids?Abundant; root-shaped

Handling information

Handling procedure
Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen.  If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week.  Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C).  Storage of frozen material at this temperature will result in the death of the culture.

1. To thaw a frozen ampoule, place in a 25–30 °C water bath, until just thawed (approximately 5 minutes).  Immerse the ampoule just sufficient to cover the frozen material.  Do not agitate the ampoule.

2. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µl (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.

3. Incubate the inoculum/strain at the temperature and conditions recommended.

4. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 3-5 days of incubation. However, the time necessary for significant growth will vary from strain to strain.  

Handling notes
No special notes.

Additional, updated information on this product may be available on the ATCC web site at

Quality control specifications

Sequenced data
No DNA sequencing was performed in house on this product.
Verification method
Whole-genome Sequencing


Deposited as
Rhizopus oryzae Went et Prinsen Geerligs, teleomorph
Rhizopus delemar (Boidin) Wehmer et Hanzawa, teleomorph; Rhizopus arrhizus Fischer, teleomorph; Rhizopus peka Takeda, teleomorph; Rhizopus boreas Yamamoto, teleomorph; Rhizopus sontii Reddi et Subramanian, teleomorph
PC Farley, G Lim
Chain of custody
P C Farley, G Lim
Type of isolate
Food & Beverage
Year of origin
Special collection
NCRR Contract

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Wibowo Y, et al. Isolation and characterization of auxotrophic mutants of Rhizopus oryzae. Cytobios 100: 95-99, 1999.

Farley PC, Sullivan PA. The Rhizopus oryzae secreted aspartic proteinase gene family: an analysis of gene expression. Microbiology 144: 2355-2366, 1998. PubMed: 9720058

Farley PC. Evaluation of the use of glucosamine measurements, for determining Rhizopus oligosporus growth during tempe fermentation. Biomed. Lett. 46: 227-228, 1991.

Farley PC, Ikasari L. Regulation of the secretion of Rhizopus oligosporus extracellular carboxyl proteinase. J. Gen. Microbiol. 138: 2539-2544, 1992. PubMed: 1487725

tempe, Singapore

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