Clavispora lusitaniae Rodrigues de Miranda
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Opportunistic pathogen for immunocompromised patients
1. To thaw a frozen ampoule, place in a 2530 °C water bath, until just thawed (approximately 5 minutes). Immerse the ampoule just sufficient to cover the frozen material. Do not agitate the ampoule.
2. Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer 10 microliter (or any amount desired up to all) of the content onto a plate or broth with medium recommended.
3. Incubate the inoculum/strain at the temperature and conditions recommended.
4. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 3-4 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Additional, updated information on this product may be available on the ATCC web site at www.atcc.org.
Francois F, et al. Alternative identification test relying upon sexual reproductive abilities of Candida lusitaniae strains isolated from hospitalized patients. J. Clin. Microbiol. 39: 3906-3914, 2001. PubMed: 11682506
human nose, Marseille, France
Noel T, et al. Flucytosine-fluconazole cross-resistance in purine-cytosine permease-deficient Candida lusitaniae clinical isolates: indirect evidence of a fluconazole uptake transporter. Antimicrob. Agents Chemother. 47: 1275-1284, 2003. PubMed: 12654658