Saccharomyces cerevisiae Meyen ex E.C. Hansen
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
1. To thaw a frozen vial, place it at room temperature or in 30°C water bath (not submerged), until just thawed (around 90 seconds).
2. Immediately after thawing, aseptically transfer the culture into a test tube or plate with medium recommended.
3. Incubate the test tube or plate at the temperature recommended (25-28°C).
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Rabitsch KP, et al. A screen for genes required for meiosis and spore formation based on whole-genome expression. Curr. Biol. 11: 1001-1009, 2001. PubMed: 11470404
derived from strain K8409 (MYA-2089) by deletion
Chromosome: 4 right arm; YDR250C