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Trace Mineral Supplement


ATCC Trace Mineral Supplement is used as an additive in many ATCC recommended media, especially for cultivating extremophiles. It speeds media preparation and reduces possible lab errors by combining several trace mineral ingredients into one sterile solution. This product is provided in an individual unit of use (10 mL).
Product category
Based on Wolfe's formulation
EDTA,  0.5 g/liter
MgSO4 . 7H2O,  3.0 g/liter
MnSO4 . H2O, 0.5 g/liter
NaCl, 1.0 g/liter
FeSO4 . 7H2O, 0.1 g/liter
Co(NO3)2 . 6H2O, 0.1 g/liter
CaCl2 (anhydrous), 0.1 g/liter
ZnSO4 . 7H2O, 0.1 g/liter
CuSO4 . 5H2O, 0.010 g/liter
AlK(SO4)2 (anhydrous), 0.010 g/liter
H3BO3, 0.010 g/liter
Na2MoO4 . 2H2O, 0.010 g/liter
Na2SeO3 (anhydrous), 0.001 g/liter
Na2WO4 . 2H2O, 0.010 g/liter
NiCl2 . 6H2O, 0.020 g/liter
10 mL
Shipping information
5 vials
Storage conditions
2°C to 8°C
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Price: $83.00 EA
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ATCC determined that a biosafety level is not applicable to this material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to complete your own risk assessment and understand any potential hazards associated with the material per your organization’s policies and procedures and any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Handling information

Handling procedure
  1. Prepare a fresh lot of medium. Sterilize by autoclaving or filter sterilizing as necessary.
  2. Remove one vial of Trace Mineral Supplement from cold storage and bring it to room temperature.
  3. If medium has been autoclaved, cool to 60°C. Aseptically add Mineral Supplement to sterile medium to the desired concentration (usually 1% but may vary according to specific formulations).
  4. Mix well and dispense medium as needed.

Quality control specifications

Bacterial and fungal testing
Not detected

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Frequently Asked Questions


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