ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
Cells contain Human papillomavirus (HPV)
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation.
This cell line is a suitable transfection host.
This cell line is useful for screening for Escherichia coli strains with invasive potential
Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.
Certified Reference Material produced under an ISO 17034 accredited process.The cells are positive for keratin by immunoperoxidase staining.
HeLa cells have been reported to contain human papilloma virus 18 (HPV-18) sequences.
P53 expression was reported to be low, and normal levels of pRB (retinoblastoma suppressor) were found.
Manufactured under an ISO 17034 accredited process
Certified Reference Material produced under an ISO 17034 accredited process.
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If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992.
AOAC International Invasiveness by Escherichia coli of mammalian cells, microbiological method. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 982.36.
Baldi A, et al. Genomic structure of the human retinoblastoma-related Rb2/p130 gene. Proc. Natl. Acad. Sci. USA 93: 4629-4632, 1996. PubMed: 8643454
Gey GO, et al. Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium. Cancer Res. 12: 264-265, 1952.
Chen TR. Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes. Cytogenet. Cell Genet. 48: 19-24, 1988. PubMed: 3180844