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Cells contain retrovirus
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
The cells express a cell surface glycoprotein of 61000 daltons (45000 dalton when not glycosylated) that is characteristic of HTLV infection.
To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.
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Essex ME, Lee TH. Method and products for detection of human T cell leukemia virus. US Patent 4,743,678 dated May 10 1988
Popovic M, et al. Isolation and transmission of human retrovirus (human t-cell leukemia virus). Science 219: 856-859, 1983. PubMed: 6600519