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HUV-EC-C [HUVEC] is an endothelial cell line that was isolated from the vein of the umbilical cord. This cell line can be used in cardiovascular disease research.
Product category
Human cells
Homo sapiens, human
Cell type
endothelial cell
Umbilical cord; Umbilical vein; Vascular endothelium
3D cell culture
Cardiovascular disease research
Product format
Storage conditions
Vapor phase of liquid nitrogen
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Detailed product information


Specific applications
This cell line is a suitable transfection host.


Growth properties
Karyology performed for one batch of CRL-1730 in 1996 reflected a hypodiploid human cell line with a modal chromosome number of 45 occurring in 72% of the cells counted, all of which had monosomic N13. The rate of polyploid cells among this population was 15.8%. This karyology differed from earlier work-ups performed on the cells that showed approximately 60% of the cells retained 2 chromosomes 13. The apparent clonal variation in cultures of CRL-1730 (most likely dependent upon passage and growth conditions) has also been noted in STR profiles with unstable alleles at D13S317 allele #9, D13S317 allele #11, and D7S820 allele #12. Other coexisting subclones include those with 46,XX,-11,-13,i(11p),i(11q) and 46,XX,+11,-13 karyotypes. For all karyotypes performed, both X chromosomes appear normal.
Yes, the cells did form colonies in semisolid medium.
No, the cells were not tumorigenic in immunosuppressed mice
Genes expressed
factor VIII
Endothelial Cell Growth Supplement (ECGS) and unidentified factors from bovine pituitary, hypothalamus or whole brain extracts are mitogenic for this line.
The cells have a life expectancy of 50 to 60 population doublings.

Handling information

Unpacking and storage instructions
  1. Check all containers for leakage or breakage.
  2. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below ­-130°C, preferably in liquid nitrogen vapor, until ready for use.
Complete medium

The base medium for this cell line is F-12K Medium (ATCC 30-2004). To make the complete growth medium, add the following components to 450 mL of the base medium:

  • 5 mL of a 10 mg/mL stock heparin solution (prepared from Sigma catalog #H3393) for a final concentration of 0.1 mg/mL heparin in complete growth medium 
    • Dissolve 1 g Heparin in 100 mL basal F-12K  and filter to make a 10 mg/mL stock solution
  • 50 mL fetal bovine serum (FBS; ATCC 30-2020)
  • 500 μL of 30 mg/mL Endothelial Cell Growth Supplement (ECGS)

Note: Because of limited stability, the ECGS should be added to an aliquot of the above culture medium fresh prior to seeding or performing fluid changes. Complete media supplemented with the below item expires 7 days after preparation.

To prepare 30 mg/mL ECGS stock solution, aseptically combine:

  • 15 mg Corning™ Endothelial Cell Growth Supplement – ECGS (ECGS; Fisher Scientific cat# CB-40006)
  • 500 µL F-12K (ATCC 30-2004)

Store in working aliquots at -20°C. ECGS is stable for 1 month when prepared and stored as directed. Do not repeat freeze/thaw.


95% Air, 5% CO2
Handling procedure

To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C.  Storage at -70°C will result in loss of viability.

  1. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water.  Thawing should be rapid (approximately 2 minutes).
  2. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
  3. Transfer the vial contents to a centrifuge tube containing  9.0 mL complete culture medium. and spin at approximately 125 x g for 5 to 7 minutes.
  4. Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio). and dispense into a new culture flask at a seeding density of 2.0 x 104 to 4.0 x 104 viable cells/cm2. It is important to avoid excessive alkalinity of the medium during recovery of the cells.  It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6). pH (7.0 to 7.6).
  5. Incubate the culture at 37°C in a suitable incubator.  A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet
Subculturing procedure

Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning T-75 flasks (catalog #430641U) are recommended for subculturing this product.

Note: A high quality ECGS prepared from bovine neural tissue (BD Biosciences catalog # 354006 or equivalent) should be used to propagate CRL-1730. It is best to initiate the cells with the highest recommended concentration of ECGS. Moderate to heavy debris and numerous floating cells may be routinely observed in cultures of HUV-EC-C cells. Retain the floating cells by gentle centrifugation and add back to the adherent population.

Cultures should be fully fluid changed every 48 hours. The cells should only be allowed to go 72 hours without fluid changing when the density is less than 50% confluent. Perform full fluid changes rather than media additions.

This cell line produces a lot of floaters and debris especially at higher densities. Cells detach before completely filling in to 100% confluence. It is recommended to subculture the cells when 80 to 90% confluent to avoid excessive floaters. Floating cells are viable and if pronounced, they should be spun down and reseeded back into the growing culture.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: Two to three times per week

Seeding Density: 8.0 x 103 to 3.0 x 104 viable cells/cm2

Reagents for cryopreservation
Complete growth medium supplemented with 5% (v/v) DMSO (ATCC 4-X)

Quality control specifications

Mycoplasma contamination
Not detected
STR profiling
D3S1358: 16
TH01: 6,9.3
D21S11: 28,31
D18S51: 13,17
D5S818: 11,12
D13S317: 9,11
D7S820: 8,12
D16S539: 11,12
CSF1PO: 11,12
vWA: 16
D8S1179: 14,16
TPOX: 8,11
FGA: 21,23
D19S433: 12,13
D2S1338: 18,22
Penta_E: 7,13
Penta_D: 12,13
Amelogenin: X


H Hoshi

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Molestina RE, et al. Characterization of a strain of Chlamydia pneumoniae isolated from a coronary atheroma by analysis of the omp1 gene and biological activity in human endothelial cells. Infect. Immun. 66: 1370-1376, 1998. PubMed: 9529055

Zahedi K. Characterization of the binding of serum amyloid P to laminin. J. Biol. Chem. 272: 2143-2148, 1997. PubMed: 8999915

Lindstrom AL, et al. An in vitro model for toxin-mediated vascular leak syndrome: ricin toxin A chain increases the permeability of human endothelial cell monolayers. Blood 90: 2323-2334, 1997. PubMed: 9310483

Soker S, et al. Inhibition of vascular endothelial growth factor (VEGF)-induced endothelial cell proliferation by a peptide corresponding to the exon 7-encoded domain VGEF165. J. Biol. Chem. 272: 31582-31588, 1997. PubMed: 9395496

Li Y, et al. Mast cell granules potentiate endotoxin-induced interleukin-6 production by endothelial cells. J. Leukocyte Biol. 62: 211-216, 1997. PubMed: 9261335

View All Curated Citations for this Product

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