Escherichia coli GFP

1. Open thawed vial according to enclosed instructions or visit www.atcc.org for instructions.
2. Aseptically transfer the entire contents to a 5-6 mL tube of #2855 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
3. Incubate while shaking at 37° C for 16 hours.
4. Although this item is ideally grown in broth, it will also grow on #2855 agar.

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