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Thermoanaerobacter uzonensis Wagner et al.


Product category
Product type
Strain designation
Type strain
Isolation source
Hot spring; isolated March 9, 2005
Geographical isolation
Russia; Kamchatka peninsula
Product format
Storage conditions
2°C to 8°C
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Biosafety Icon Biosafety Level 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information




Extremophile type

Handling information

Handling procedure
  1. Sterilize the top of the all test tubes by spraying with 70% ethanol and then flaming the tops.
  2. If needed, exchange the gas in the test tube for 80% N2 20% CO2 or 100% N2.
  3. If the medium is pink add 0.2 ml Co-enzyme M (5% stock solution) per 10 ml of medium.  Let the medium sit at room temperature for 30 to 40 minutes until the medium becomes colorless before inoculating.
  4. When the medium is ready to inoculate, open the vial according to enclosed instructions.
  5. For inoculation, use an anaerobic 1.0 ml syringe (see below) tipped with 22 gauge needle.  Withdraw 0.5 ml of #2682 broth and use this to rehydrate the entire freeze dried pellet.  Immediately place the rehydrated vial under a stream of sterile oxygen-free gas. 
  6. Using the same syringe, transfer the rehydrated cell suspension back to the tube of #2682 broth.  Plate 0.1 ml of the inoculated culture onto a non-selective medium and incubate aerobically at 30oC.  Use 0.1 ml of the inoculated culture to inoculate a nonselective aerobic broth.  Transfer 0.5 ml of the culture to an additional tube of #2682 broth. Incubate the broth tubes at 60oC. 
  7. Growth should be detected in the #2682 broth within 24 to 48 hours.  There should be no growth detected on the aerobic plate or in the aerobic broth.
Handling notes
Cells are gram-negative motile rods that occur in pairs and chains.  Minimal growth on ATCC® media #260 blood agar occurs with colonies being circular, transparent, convex and glistening.

Additional information on this culture is available on the ATCC© web site at



J Wiegel, WD Isaac
Type of isolate
Year of origin
Cross references
GenBank EF530067 Thermoanaerobacter uzonensis strain JW/IW010 16S ribosomal RNA gene, partial sequence

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Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


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Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

Wagner, et al. Thermoanaerobacter uzonensis sp. nov., an anaerobic thermophilic bacterium isolated from a hot spring within the Uzon Caldera, Kamchatka, Far East Russia. Int. J. Syst. Evol. Microbiol. 58(Pt 11): 2565-2573, 2008. PubMed: 18984694

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