Chlamydia trachomatis Serovar E (ATCC® VR-348B™) Classification: Chlamydiaceae / Product Format: frozen 1 mL per vial General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Classification Chlamydiaceae Deposited As Chlamydia trachomatis (Busacca) Rake Agent Chlamydia trachomatis Serovar E Strain BOUR Applications Sexually Transmitted Disease Research Biosafety Level 2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Product Format frozen 1 mL per vial Storage Conditions -70°C or colder Comments FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.Rapid loss in titer when stored above -70°C. Activities with high potential for aerosol production require BSL 3 facilities and practices. Effect on Host Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated monoclonal antibodies Recommended Host McCoy (ATCC® CRL-1696™) Growth Conditions Temperature: 35.5°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 90-100% confluent. Prior to infection, disrupt cells by sonication or vortex with sterile glass beads. Remove medium and inoculate with a small volume of inoculum (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 1). Adsorb by centrifugation at 2100 x g for 1 hour at 20°C. End adsorption by adding growth medium. Incubation: 3 days Effect on Host Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated monoclonal antibodies Name of Depositor L Hanna, E Jawetz, Centers for Disease Control and Prevention Special Collection NCRR Contract Source Adult with probable clinical inclusion conjunctivitis and active trachoma, California, 1959 References Hanna L, et al. Elementary-body virus isolated from clinical trachoma in California. Science 130: 1339-1340, 1959. PubMed: 14399528 Basic Documentation Product Sheet Certificate of Analysis SDS Other Documentation Microbial Characterization Data