Human respiratory syncytial virus (ATCC® VR-1580)

Classification: Paramyxoviridae, Pneumovirus  /  Product Format: frozen 1 mL per vial

Classification Paramyxoviridae, Pneumovirus
Deposited As Respiratory syncytial virus
Agent Human respiratory syncytial virus
Strain 18537
Applications
Respiratory research
Positive for Mycoplasma No
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1580 images
Comments The early passage history of this item is unknown.
Effect on Host
CPE, massive syncytia formation and sloughing
Recommended Host
HEp-2 (ATCC® CCL-23™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3-7 days
Effect on Host
CPE, massive syncytia formation and sloughing
Name of Depositor RM Chanock, J Crowe, National Institute of Allergy and Infectious Diseases; ATCC®
Special Collection ATCC
Source Respiratory secretions from child with acute respiratory disease seen at Children’s Hospital of the District of Columbia, Washington, DC, 1962. Treated ATCC® VR-1401™ to remove mycoplasma at ATCC®.
References

Coates HV, et al. An antigenic analysis of respiratory syncytial virus isolates by a plaque reduction neutralization test. Am. J. Epidemiol. 83: 299-313, 1966. PubMed: 5933417