Human genomic DNA (HCC1954) (ATCC® CRL-2338D)

Organism: Homo sapiens, human  / 

Product Format frozen Approx.10 µg
Storage Conditions -20°C or colder in a manual defrost freezer. Avoid freeze-thawing.
Biosafety Level 1
Introduction

Cell Line Description:

The HCC1954 line is a poorly differentiated cell line initiated on October 30, 1995; it took about 4 months to establish.  HCC1954 was derived from a primary stage IIA, grade 3 invasive ductal carcinoma with no lymph node metastases.  HCC1954 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 and for cytokeratin 19, and is negative for  expression of estrogen receptor (ER) and progesterone receptor (PR). Her2/neu is overexpressed in the ELISA assay.  DNA isolated from an EBV-transformed lymphoblastoid cell line, HCC1954 BL, from the same patient is available as ATCC CRL-2339D.

Quality Control Specifications

DNA Quality Control Information:

·         > 100 kb in size by agarose gel electrophoresis

·         No RNA detected in the agarose gel

·         A260/A280 ratio ³ 1.8

·         Tested and verified for PCR amplification and restriction digestion.