C-4 II (ATCC® CRL-1595)

Organism: Homo sapiens, human  /  Tissue: cervix  /  Disease: carcinoma

Organism Homo sapiens, human
Tissue cervix
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 [Cells contain human papilloma virus]
Disease carcinoma
Age 41 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Derivation
This is one of two lines developed from one patient (see ATCC CRL-1594).
Clinical Data
female
Caucasian
41 years
Comments
The line contains human papillomavirus type 18 (HPV-18) DNA sequences, and expresses HPV-18 RNA.
Complete Growth Medium Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
Subculturing
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 3 times per week
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 11,12
D16S539: 11
D5S818: 9,11
D7S820: 10,11
THO1: 9,9.3
TPOX: 10,11
vWA: 14
Isoenzymes
G6PD, B
Name of Depositor N Auersperg
References

Auersperg N, Hawryluk AP. Chromosome observations on three epithelial-cell cultures derived from carcinomas of the human cervix. J. Natl. Cancer Inst. 28: 605-627, 1962. PubMed: 13863218

Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217

Auersperg N. Histogenetic behavior of tumors. I. Morphologic variation in vitro and in vivo of two related human carcinoma cell lines. J. Natl. Cancer Inst. 43: 151-173, 1969. PubMed: 5796380

Auersperg N. Histogenetic behavior of tumors. II. Roles of cellular and environmental factors in the in vitro growth of carcinoma cells. J. Natl. Cancer Inst. 43: 175-190, 1969. PubMed: 5796382

Auersperg N. Histogenetic behavior of tumors. 3. Possible relationships to patterns of glycolysis. J. Natl. Cancer Inst. 48: 1589-1596, 1972. PubMed: 5056250

Lee HC, Auersperg N. Calcium in epithelial cell contraction. J. Cell Biol. 85: 325-336, 1980. PubMed: 6768754

Basic Documentation
References

Auersperg N, Hawryluk AP. Chromosome observations on three epithelial-cell cultures derived from carcinomas of the human cervix. J. Natl. Cancer Inst. 28: 605-627, 1962. PubMed: 13863218

Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217

Auersperg N. Histogenetic behavior of tumors. I. Morphologic variation in vitro and in vivo of two related human carcinoma cell lines. J. Natl. Cancer Inst. 43: 151-173, 1969. PubMed: 5796380

Auersperg N. Histogenetic behavior of tumors. II. Roles of cellular and environmental factors in the in vitro growth of carcinoma cells. J. Natl. Cancer Inst. 43: 175-190, 1969. PubMed: 5796382

Auersperg N. Histogenetic behavior of tumors. 3. Possible relationships to patterns of glycolysis. J. Natl. Cancer Inst. 48: 1589-1596, 1972. PubMed: 5056250

Lee HC, Auersperg N. Calcium in epithelial cell contraction. J. Cell Biol. 85: 325-336, 1980. PubMed: 6768754