Trichoderma reesei Simmons (ATCC® 56765)

Strain Designations: NRRL 11460 [NRCC 2906, RUT-C30, VTT-D-86271]  /  Product Format: freeze-dried

Strain Designations NRRL 11460 [NRCC 2906, RUT-C30, VTT-D-86271]
Produces acetylesterase acetyl esterase
Produces alkaline xylan endo-1,3-beta-xylosidase alkaline xylanase
Produces alpha-L-arabinofuranosidase alpha-1,3-arabinosidase
Produces alpha-galactosidase
Produces alpha-glucuronidase
Produces cellobiohydrolases I and II
Produces endoglucanase
Produces exo-cellobiohydrolase avicelase, endoglucanase I
Produces lactase
Produces mannan endo-1,4-beta-mannosidase beta-mannanase, mannanase
Produces polygalacturonase endopolygalacturonase, pectinase
Produces xylan endo-1,3-beta-xylosidase xylan hydrolase, xylanase
Transformation host
Produces beta-xylanases I and II
Produces cellulase-poor xylanases
Enhancement of cellulase production with citric acid
Transformation host for expression of Phlebia radiata laccase gene
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Preceptrol® no
Genome Sequenced Strain


Ethanol- and polyene-resistant
Genome sequencing strain (the Joint Genome Institute at the Department of Energy, USA).
Morphology After 6 days on Potato Dextrose agar at 25°C, colony is low, velutinous, mycelium white, conidia blue-green en masse; reverse vibrant yellow. Conidia ovoid, green, smooth-walled.
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence


D1D2 region of the 28S ribosomal RNA gene


Morphology After 6 days on Potato Dextrose agar at 25°C, colony is low, velutinous, mycelium white, conidia blue-green en masse; reverse vibrant yellow. Conidia ovoid, green, smooth-walled.
Name of Depositor NRRL
Chain of Custody
ATCC <-- NRRL <-- Rutgers Univ. RUT-C30
Isolation Not available.
Cross References

Nucleotide (GenBank) : KU729092 ITS including 5.8S rRNA gene

Nucleotide (GenBank) : KU729195 D1/D2 region of 28S rRNA gene

Nucleotide (GenBank) : X93938 T.reesei rRNA genes and ITS1 and ITS2 DNA (strain ATCC 56765


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Kurzatkowski W, et al. Ultrastructural localization of cellular compartments involved in secretion of the low molecular weight, alkaline xylanase by Trichoderma reesei. Arch. Microbiol. 159: 417-422, 1993.

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Zhang L, et al. A novel host-vector system for heterologous protein co-expression and purification in the Trichoderma reesei industrial strain RUT-C30. Biotechnol. Lett. 38: 89-96, 2016. PubMed: 26343029

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