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Chlamydophila pneumoniae (ATCC® 53592)

Classification: Chlamydiaceae, Chlamydophila  /  Product Format: frozen 1 mL per vial

Permits and Restrictions

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Classification Chlamydiaceae, Chlamydophila
Deposited As Chlamydia pneumoniae
Agent Chlamydophila pneumoniae
Strain AR-39
Applications
Respiratory research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Comments Activities with high potential for aerosol production require Biosafety Level 3 facilities and practices. The inclusions are iodine stain negative (contain no glycogen). The TWAR strains of Chlamydia are named after TW183 and AR39. This material is cited in a U.S. and/or other Patent Application and may not be used to infringe the patent claims.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa.
Recommended Host
HEp-2 (ATCC® CCL-23™)
Growth Conditions

Temperature: 35°C

Recommendations for Infection: Plate cells 16-24 hours prior to infection and infect when cultures are 80-90% confluent. Disrupt cells in the inoculum by sonicating for 20 seconds at approximately 240W or by adding glass beads to the inoculum and vortex mixing. Remove medium and inoculate with disrupted material. Centrifuge at 2,300 - 2,400 x g at 20°C for 1 hour. End adsorption by adding agent growth medium.

Incubation: 3 days at 35°C in a humidified 5% CO2 atmosphere.

Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa.
Name of Depositor Washington Research Foundation
U.S. Patent
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Special Collection NCRR Contract
Source Throat of university student with acute pharyngitis, Seattle, WA, 1983
References

Grayston JT, et al. Chlamydia pnemoniae sp. nov. for Chlamydia sp. Strain TWAR. Int. J. Syst. Bacteriol. 39:88-90, 1989.

Kuo CC, Grayston JT. Chlamydia spp. strain TWAR: A newly recognized organism associated with atypical pneumonia and other respiratory infections. Clin. Microbiol. Newsletter 10: 137-140, 1988.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation