Genomic DNA isolated from a preparation of Clostridioides difficile strain 1470 (ATCC® 43598™). The source organism is also available through the ATCC catalog.
Presence of tcdB
gene confirmed by PCR RefLemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303
Binary toxin gene cdtB not amplified by PCR