Xylella fastidiosa subsp. fastidiosa Wells et al.

1. Open vial according to enclosed instructions.                            
2. Using a single tube of #1099 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette.  Rehydrate pellet. 
3. Aseptically transfer this aliquot back into the broth tube.  Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate. 
5. Incubate all tubes and plate at 26°C for 14 days.

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